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microbiology

Quiz yourself by thinking what should be in each of the black spaces below before clicking on it to display the answer.
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Question
Answer
show a gain of electron or hydrogen atoms  
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show skin normal flora makes catalase  
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differentiate between aerobic and anaerobic respiration   show
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show Fermentation- organic compound is final electron acceptor Anaerobic respiration- inorganic compounds other than O2 are final electron acceptor  
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Would nitrate reduction occur more often in the presence or absence of O2?   show
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In nitrate reduction test, what does the presence of gas indicate?   show
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show nitrate reduction is harmful because plants need nitrates to survive. If nitrates are reduced to NO or N2, there is less available for the plants.  
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show The nitrates were reduced to nitrites then nitrous oxide and finally to nitrogen gas  
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When did E. faecalis exhibit color change in the nitrate reduction test?   show
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show To classify bacteria as aerobic or anaerobic.  
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show P. aeruginosa and B. subtilis  
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What organism was positive and what organism was negative for the oxidase test?   show
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show E. faicalis and B. subtilis  
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What organism was positive in the Catalase test?   show
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show anaerobes  
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The oxidase and catalase test test for what kind of organisms?   show
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show If there is gas, the nitrate was reduced to nitrogen gas.  
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show nitrate-->nitrite-->nitrous oxide-->nitrogen gas  
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show 3,000,000  
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The DNA of E. coli is attached to what?   show
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What is the average fragment length of the large fragments of chromosomal DNA?   show
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what is the average length of a gene?   show
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A convenient method for analyzing DNA of e. coli that consists of microscopic pores in the gel that act as a molecular sleeve   show
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where are the samples of DNA loaded into in the Agarose gel?   show
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show DNA has a strong negative charge at neutral pH  
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show The pores in the gel separate the linear fragments according to their size  
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Why does DNA appear as a smear?   show
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What is the objective of the Isolation of E.coli DNA lab?   show
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show EDTA  
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What forms complexes (chelates) with several kinds of metal ions?   show
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show Mg+2  
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what dissolves the cell membrane and denatures many proteins?   show
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show RNase  
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show protease  
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show protease  
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show 5ml  
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show EDTA  
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how many different bacteria were in the mixed culture in isolation by dilution?   show
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How many bacteria were in the turbid broth in isolation by dilution?   show
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In isolation by dilution...how do colonies on the surface of the pour plates differ from those suspended in agar?   show
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what is a contaminate?   show
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show it was in an area that was uninoculated and has different morphology  
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show bacteria would be overgrown  
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What would happen if plates were incubated a month longer?   show
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How could your streak plate technique be improved?   show
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show depends on O2 requirements  
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show too many to count  
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show hard to see isolation and solidifies too fast  
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What three organisms did we use in the oF-glucose test?   show
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show carbon and energy  
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What three sizes are carbohydrates classified as?   show
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How do we look for the presence of an exoenzyme?   show
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show glucose  
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What are the metabolic endproducts of fermentation?   show
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What test helps determine whether an organism is oxidative or fermentative?   show
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What indictor does OF glucose use?   show
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bromtymol blue turns what color in the presence of acids?   show
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