Quiz One - Genetics Fall 2007
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
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| What is the cell structure present in eukaryotic cells and absent from prokaryotic cells | Nucleus
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| What is the nucleolus made of | mRNA
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| What is the unit of hereditary which may produce multiple proteins | Gene
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| What is the function of the Golgi apparatus | post translational protein modification
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| Which key protein accepts cell membrane signals and transfers them to internal messenger systems by activating cytoplasmic enzymes | G protein
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| Which mitotic phase has chromosomes lining up along the equatorial plate | Metaphase
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| What is the outcome of meiosis | Daughter cells with N ploidy
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| What is the unique step in meiosis which produces the phenotype variety in offspring | Crossover (Recombination)
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| What is one of a family of two or more genes that may occupy a locus | Allele
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| What is an epigenetic change | Modification of a gene such as methylation that silences the gene
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| How much of the human genome is non-coding | 95%
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| On mRNA, what is the poly-A region | A sequence of up to 200 adenines on the 3' end
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| What is the promotor sequence | AUG (methionine)
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| Name the stop codons | UAG UAA UGA
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| What form of RNA binds an amino acid and attaches it to the growing polypeptide chain in response to a specific codon | Transfer RNA (tRNA)
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| What is the unexpressed portion of an eukaryotic gene | Intron
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| In many bacteria, there is a portion of DNA seperate from the genomic DNA, what is it called? | Plasmid
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| DNA is the template for heterogenous nuclear RNA (hnRNA). What is the term for hnRNA production? | Transcription
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| What is the base that is complementary to adenine, and how many hydrogen bonds hold them together | Thymine and two hydrogen bonds
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| Which enzyme catalyzes the extension to the daughter DNA molecule upon the template | DNA polymerase
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| What is a limitation of ethidium bromide dye | Carcinogen (mutagen)
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| What is a good way to store extracted DNA for an extended period of time | Freeze at -70 degrees centigrade
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| In the Qiagen solid phase precipitation method for DNA extraction, what is the medium the DNA binds during separation | Silica column
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| In the PureGene organic method for DNA extraction what solvent causes DNA to precipitate | Isopropanol
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| What kind of human specimen is best for calculating viral load | Serum or plasma
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| SDS purpose | In PAGE is to denature to make into smaller fragments
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| Capillary electorphoresis | is automated as in gene sequencing
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| Restriction endonucleases are named as | enzymes that restrict phage infections of E. Coli
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| What are restriction enzymes used for? | RFLP
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| Southern Blot | DNA separated by gel electophoresis by transferring the bands from gel to nylon then treating with a probe.
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| Vacuum or electo-blotting | technique used to speed up band transfer when performing Southern Blot
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| Western blot | electrophoresis blot method that analyses proteins and polypeptides.
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| Denature DNA with NaOH before transferring to membrane | Method used to support probe hybridization in Southern Blot
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| What is a Variable Number Tandem Repeat? | Untranslated repeated base pair sequences up to 100 bp
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