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Quiz One
Quiz One - Genetics Fall 2007
| Question | Answer |
|---|---|
| What is the cell structure present in eukaryotic cells and absent from prokaryotic cells | Nucleus |
| What is the nucleolus made of | mRNA |
| What is the unit of hereditary which may produce multiple proteins | Gene |
| What is the function of the Golgi apparatus | post translational protein modification |
| Which key protein accepts cell membrane signals and transfers them to internal messenger systems by activating cytoplasmic enzymes | G protein |
| Which mitotic phase has chromosomes lining up along the equatorial plate | Metaphase |
| What is the outcome of meiosis | Daughter cells with N ploidy |
| What is the unique step in meiosis which produces the phenotype variety in offspring | Crossover (Recombination) |
| What is one of a family of two or more genes that may occupy a locus | Allele |
| What is an epigenetic change | Modification of a gene such as methylation that silences the gene |
| How much of the human genome is non-coding | 95% |
| On mRNA, what is the poly-A region | A sequence of up to 200 adenines on the 3' end |
| What is the promotor sequence | AUG (methionine) |
| Name the stop codons | UAG UAA UGA |
| What form of RNA binds an amino acid and attaches it to the growing polypeptide chain in response to a specific codon | Transfer RNA (tRNA) |
| What is the unexpressed portion of an eukaryotic gene | Intron |
| In many bacteria, there is a portion of DNA seperate from the genomic DNA, what is it called? | Plasmid |
| DNA is the template for heterogenous nuclear RNA (hnRNA). What is the term for hnRNA production? | Transcription |
| What is the base that is complementary to adenine, and how many hydrogen bonds hold them together | Thymine and two hydrogen bonds |
| Which enzyme catalyzes the extension to the daughter DNA molecule upon the template | DNA polymerase |
| What is a limitation of ethidium bromide dye | Carcinogen (mutagen) |
| What is a good way to store extracted DNA for an extended period of time | Freeze at -70 degrees centigrade |
| In the Qiagen solid phase precipitation method for DNA extraction, what is the medium the DNA binds during separation | Silica column |
| In the PureGene organic method for DNA extraction what solvent causes DNA to precipitate | Isopropanol |
| What kind of human specimen is best for calculating viral load | Serum or plasma |
| SDS purpose | In PAGE is to denature to make into smaller fragments |
| Capillary electorphoresis | is automated as in gene sequencing |
| Restriction endonucleases are named as | enzymes that restrict phage infections of E. Coli |
| What are restriction enzymes used for? | RFLP |
| Southern Blot | DNA separated by gel electophoresis by transferring the bands from gel to nylon then treating with a probe. |
| Vacuum or electo-blotting | technique used to speed up band transfer when performing Southern Blot |
| Western blot | electrophoresis blot method that analyses proteins and polypeptides. |
| Denature DNA with NaOH before transferring to membrane | Method used to support probe hybridization in Southern Blot |
| What is a Variable Number Tandem Repeat? | Untranslated repeated base pair sequences up to 100 bp |
Created by:
mlynnm727
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