Question | Answer |
what is a specialized element? | capsid |
viruses are best observed by..... | TEM |
what kind of staining is used in TEM | negative staining |
viral envelope is composed of... | lipid membrane and viral protein embedded |
a nucleocapsid is... | a naked virus |
how many structual types of viruses are there? | 1. icosahedral
2. helical
3. complex |
envoloped virus consist of... | nucleocapsid and envelope |
smallest virus is a... | parovirus |
genus = ? | virus |
family = ? | viridae |
order = ? | virales |
isosahedron is... | 20 sides, equilateral triangles on each side, 12 corners, and 30 edges... similar to a sphere. |
2 fold symmetry for a icosahedron is... | can rotate at 180 degrees and have the same orientation from the side edge |
3 fold symmetry for a icosahedron is... | through the middle has equal symmetry |
5 fold symmetry for a icosahedron is... | folding through a corner |
intracapsomeric bond and intercapsomeric bonds are what kind of bonding? | non covalent bonds |
which one is stronger bonding? inter or intra- caposmeric? | intracapsomeric |
an example of a complex virus structure | poxvirus |
what determines virus structure? | protein protein interaction, protein and NA interaction, protein structure, protein sequence, viral gene sequence |
virus that contain GLUE (matrix protiens) | orthoyxoviridae, pamyxovirdae, rhabdoviridae |
what type of viruses are sensitive to bile and acid? | enveloped viruses |
ebola virus | filoviridae |
rabies virus | rhabdoviridae |
rota virus | reoviridae |
common cold | rhinoviruses,adenoviridae, picornaviridae |
rubella | togaviridae |
yellow fever and west nile virus | flaviviridae |
SARS | coronaviridae |
Influenza | orthomyxoviridae |
polio | picornaviridae |
measles | paramyxoviridae |
chickenpox, herpes, EBV | herpesviridae |
hanta virus | bunyaviridae |
lassa fever | arenaviridae |
primary culture is... | 1st set of cells directly frm a animal and cultured and cells are growing |
primary cell line is... | primary culture being subcultured that are diploid |
continuous cell line... | ready supply of immortal aneuploid cells |
types of assay... | infectivity and physico-chemical |
types of infectivity assay... | quantal and quantitative |
quantal assay consist of... | recording the highest dilution that is causing CPE |
a colony represents a.... | specific strain; pure culture |
one hit kinetics | one infectious virus is suffient to form a plaque |
two hit kinetics | 2 virus particles is suffient to form a plaque |
particle to infectivity ratio (PI)... | total # of particles per infectious sites |
Efficiency of plating (EOP) | # of infectious units per total # of particle |
multiplicity of infection (MOI) | m = -ln P(o) |
one step growth expt is done by | max dulbruck |