Question | Answer |
Which step in homologous recombination is catalyzed by RecA or Rad51? | Strand invasion |
What protein cleaves in the junction to resolve holliday junction in bacteria? | RuvC |
Which step in homologous recombination is catalyzed by RuvAB? | Branch migration |
Which protein creates double stranded breaks in DNA during meiosis in eukaryotes? | Spo11 |
During meiosis crossing over occurs between | non-sister chromatids |
In yeast mating type switching the the HO endonuclease | Cleaves specifically at the MAT locus |
A classic example of site-specific recombination is | Bacteriophage lambda integration |
Site specific recombination between two recombination recognition sequences (RRS) which are in direct orientation with respect to each other on a single double stranded DNA will result in _______ of the DNA between RRS | Deletion |
Cre-LoxP system for generating knock-out mice is an example of | Site specific recombination |
Transposons are | transposable elements and segments of RNA |
The ends of a simple transposable element contain | inverted repeats |
Which of the following utilizes and RNA intermediate of transposition | Retrotransposons |
Human L1 and Alu are examples of | transposons |
The V, J, and D segments.. | Are each selected randomly |
The enzyme that accomplishes transcription is termed | RNA polymerase |
The sigma subunit of bacterial RNA polymerase | Recognizes promoter sites in the DNA |
The RNA polymerase that produces mRNA molecules in eukaryotic cells is | RNA polymerase II |
The first protein complex to bind to the core promoter for a protein-coding gene in eukaryotes | General transcription factor TFIID |
Which modification must be made to RNA polymerase II in order to activate the pre initiation complex | phosphorylation |
Which of the following statements are true about eukaryotic mRNA?
A cap is added to their 5' end, A poly-A tail is added to their 3' end, each usually specifies only a single protein | All of them |
Processing of pre-mRNA to make mRNA involves | Using a spliceosome to cut out the introns |
Splicing joins together | two exons |
Which of the following do snRNPs bind to | 5' and 3' end of the intron |
The 3' splice site contains a GU, whereas the 5' splice site contains an AG | False; Reversed |
What statement correctly describes trans-splicing | Exons from different RNA transcripts are joined together |
Which of the following is an example of RNA editing | Alteration of the nucleotide sequence of an RNA molecule |
The protein encoded by Drosophila doublesex (Dsx) gene is regulated by | Alternate splicing |
According to the holliday model, the first step of recombination involves a nick in | only one DNA strand of each of the two homologous chromosomes |
After isomerization in Holliday model, cleavage by an endonuclease results in | two recombinant chromosomes, each containing a heteroduplex |
Which of the following sites is a hot spot for recombination in bacteria | Chi |
What typically initiates homologous DNA recombination | random double-stranded break in duplex DNA |