Question | Answer |
If the (delta)H is lower than 0, the reaction is... | Exothermic |
If the (delta)H is greater than 0, the reaction is... | Endothermic |
What is the first law of thermodynamics? | Energy cannot be created or destroyed, only transferred |
What is the 2nd law of thermodynamics? | A system tends to maximum entropy/disorder |
Gibbs free energy equation with deltaH and deltaS | deltaG = deltaH - T.deltaS |
If deltaG is less than 0, the reaction.. | Will occur spontaneously |
If deltaG is greater than 0 the reaction.. | Will not occur spontaneously |
If deltaG is 0, the reaction.. | Is at dynamic equilibrium |
How is deltaG related to Keq? | deltaG = -R.T.Ln(Keq) |
What does coupling reactions allow? | Allows unfavourable reactions (deltaG > 0) to occur by coupling with a favourable one (deltaG<0) |
Enzyme classification, what is 1? | Oxidoreductase |
Enzyme classification, what is 2? | Transferase |
Enzyme classification, what is 3? | Hydrolysases |
Enzyme classification, what is 4? | Lysases |
Enzyme classification, what is 5? | Isomerase |
Enzyme classification, what is 6? | Ligases |
If a enzymatic product changes colour, how could we measure rate? | Using a spectrophotometer |
Why do we use a line weaver burk plot? | Because Michaelis-menten plot can produce noisy data |
What does the Km tell us about how good a substrate is for an enzyme? | The lower the Km, the better the substrate is for an enzyme |
How does Vmax and Km relate to the initial rate | Vo = (Vmax.[S]) / (Km + [S]) |
How does a competitive inhibitor affect the michaelis menten plot? | Vmax remains the same, Km increases |
How does a competitive inhibitor affect a lineweaver burk plot? | C stays the same, m changes |
How does an uncompetitive inhibitor affect the michaelis menten plot? | Vmax decreases, Km decreases |
How does an uncompetitive inhibitor affect the lineweaver burk plot? | m stays the same, C is changing |
How does a non-competitive inhibitor affect a michaelis menten plot? | Vmax decreases, Km decreases |
How does a non-competitive inhibitor affect a lineweaver burk plot? | It can do anything |
How does substrate inhibition for an enzyme work? | Final product inhibits the enzyme, slowing down the RDS |
What enzyme catalysts the addition of phosphate group? | Kinase |
What enzyme catalyses dephosphorylation? | Phophatases |
What are the three chemical composition of hormones? | Peptide/protein hormones
Biogenic amines
Steroid |
What enzyme turns ATP to cAMP? | |
What enzyme is used in the phosphoinisitide pathway? | Phosolipase C (PLC) |
What does Phospholipase (PLC) do? | Splits PIP2 into DAG and InsP3 |
What binds to the calcium channels on the endoplasmic reticulum in the phosphoinositide pathway? | InsP3 |
How does insulin increase glucose uptake? | Vehicle underneath the cell membrane full of glucose transporters fuses with the cell membrane |