| Question | Answer |
| Transduction | Transfer of bacterial genetic info using bacteriophage.
Generalized and specialized |
| Transposition | insertion of genetic information into genome
may carry additional info
disrupt normal gene or insert new info |
| Intracellular bacterial infection | Th1 mediated DTH, CTLs and ADCC |
| Extracellular bacterial infection | Th2 ab mediated response;
opsonization, C', ADCC |
| Innate response to Bacteria | Inflamation - phagocytic cells, C'
Mac lysis if unencapsulated Gram -/ cell wall-less |
| Id Ag specific T cells | MHC class I tetramers;
bound to biotinylated avidin |
| Cytotoxicity Assay | 51Cr release assay;
51Cr added to infected target cell lysed? by CD8;
measure release --> effective killing? |
| Microtoxicity assay | Trypan blue test;
Anti-HLA for MHCI; |
| TGF-beta | secreted by platelets, macrophages, lymphocytes and mast cells. Fxn: stimulate epithelial and mesenchymal growth. Downregulates proinflamatory activities. Induces IgA class switching |
| IL-1 | •Secreted by mono, macro, dendritic, endothelial, B and other •major role in the development of innate response |
| IL-2 | •Secreted by activated T cells |
| IL-3 | •Secreted by T cells, NK cells, eosinophils and mast cells |
| IL-4 | •Secreted by T cells, NK cells and mast cells– Regulates some stages of hematopoiesis |
