Question | Answer |
partition coefficient | lower= will pass through the membrane (lipid soluble) |
chloroform-methanol | lipid solvent |
Triton X-100 | nonionic detergent |
Sodium dodecyl sulfate | ionic detergent |
most water soluble compound in list | n-propanol |
least water soluble compound in list | glycerol |
5 properties that make E. coli DH5-alpha safe | pro/thi auxotroph, no polysaccharide layer, cell wall defects, lack of enzyme that degrades plasmid DNA, lack of ability to recombine DNA |
EDTA | destabilizes cell wall and protects DNA from degradation |
restriction enzymes depend on (2) | Mg2+ ions and neutral pH |
find ug DNA | absorbance at 260 (DNA) x 50 ug/mL x 15 dilution x 1mL/1000 uL x 50 uL |
absorbance is directly proportional to | concentration of the sample |
hypotonic | less solutes outside of cell, cell bursts |
bacterial trait helpful to molecular biologist but not doctor | altered so it cannot make one sick |
the two different types of DNA present in bacteria are similar in what sense? | double stranded, encode for genes |
wavelength DNA | 260nm |
wavelength protein | 280nm |
how did we differentiate between transformed and non-transformed cells? | whether or not they grew on antibiotics |
two types of competent cells | yeast (naturally), any cell treated w/ CaCl2 |
crenate | hypertonic |
calculate CFU/mL | colonies/mL/(1/dilution) |