| Term | Definition |
| Ferric Iron | Fe 3+
Prussian Blue
(equal parts potassium ferrocyanide and Hydrochloric acid)
naturally found in small amounts in the bone marrow and spleen |
| Ferrous Iron | Fe 2+
Turnbull Blue
(potassium Ferricyanide w/ hydrochloric acid .4g to 40ml of .06N solution)
used in the schmorl technique |
| Prussian Blue | for ferric iron
equal parts potassium ferocyanide and hydrochloric acid
counterstain with nuclear fast red |
| Prussian Blue | Nuclei & Hemofuchsin.....BRIGHT RED
Hemosiderin (iron)....... BLUE
Background...................PINK |
| Turnbull Blue | for ferrous iron
ferrocyanide solution
wash in acetic acid |
| Turnbull Blue | Ferrous Iron...... BLUE
background......PINK/RED |
| Churukian-Schenk | method for argyrophil granules
demonstrates argyrophil granules in neurosecretory tumors |
| Churukian-Schenk | argyrophil and argentaffin....BLACK
nuclei......................RED
background................YELLOW/BROWN |
| Grimelius | for Argyrophil granules in neurosecretory tumors
hydroquinone and sodium sulfite used to reduce silver nitrate solution |
| Von Kossa | stain for calcium
Silver reacts with the anions (carbonate and phosphate) of calcium salts
a bright light is used to reduce the silver |
| Von Kossa | calcium salts........BLACK
background.........RED |
| Allzarin Red S | stain for calcium
place in allzarin red s solution and check progress microscopically |
| Allzarin Red S | calcium deposits...ORANGE/RED |
| Schmorl Technique | used to demonstrate reducing substances such as :
melanin, argentaffin granules, formalin pigment
ferric chloride-potassium ferricyanide produces turnbull blue precipitate
mayer mucicarmine
metanil yello |
| Schmorl Technique | reducing substances...........BLUE/GREEN
goblet cell-mucin..............ROSE
background.....................YELLOW-GREEN |
| Fontana Masson | demonstrates melanin and argentaffin granules
argentaffin granules reduce silver naturally
silver nitrate->gold chloride -> sodium thiosulfate -> nfr |
| Fontana Masson | Melanin.............................BLACK
Argentaffin Granules..........BLACK
Nuclei................................PINK |
| Rhodanine method | demonstrates copper
detects copper especially in liver as in Wilson's disease
rhodanine solution-> mayor hematoxilyn-> sodium borate |
| Rhodanine method | copper.......BRIGHT RED/YELLOW
nuclei.........LIGHT BLUE |
| Gomori Methenamine Silver | demonstrates urates as found in gout
deparaffinize and rinse with absolute alcohol->methenamine silver->sodium thiosulfate->light green counter |
| Gomori Methenamine Silver | urates.................BLACK
background........BLUE/GREEN |
| Hall Stain for Bile | demonstrates bilirubin and can distinguish bile pigment from other pgments
green color develops when the bilirubin oxidizes with ferric chloride in trichloroacetic acid medium called FOUCHET REAGENT
*counter with van giesen (acid fuchsin/picric acid) |
| Halll Stain for Bile | bilirubin................GREEN
background...........YELLOW |
