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special stains
Minerals and Pigments
| Term | Definition |
|---|---|
| Ferric Iron | Fe 3+ Prussian Blue (equal parts potassium ferrocyanide and Hydrochloric acid) naturally found in small amounts in the bone marrow and spleen |
| Ferrous Iron | Fe 2+ Turnbull Blue (potassium Ferricyanide w/ hydrochloric acid .4g to 40ml of .06N solution) used in the schmorl technique |
| Prussian Blue | for ferric iron equal parts potassium ferocyanide and hydrochloric acid counterstain with nuclear fast red |
| Prussian Blue | Nuclei & Hemofuchsin.....BRIGHT RED Hemosiderin (iron)....... BLUE Background...................PINK |
| Turnbull Blue | for ferrous iron ferrocyanide solution wash in acetic acid |
| Turnbull Blue | Ferrous Iron...... BLUE background......PINK/RED |
| Churukian-Schenk | method for argyrophil granules demonstrates argyrophil granules in neurosecretory tumors |
| Churukian-Schenk | argyrophil and argentaffin....BLACK nuclei......................RED background................YELLOW/BROWN |
| Grimelius | for Argyrophil granules in neurosecretory tumors hydroquinone and sodium sulfite used to reduce silver nitrate solution |
| Von Kossa | stain for calcium Silver reacts with the anions (carbonate and phosphate) of calcium salts a bright light is used to reduce the silver |
| Von Kossa | calcium salts........BLACK background.........RED |
| Allzarin Red S | stain for calcium place in allzarin red s solution and check progress microscopically |
| Allzarin Red S | calcium deposits...ORANGE/RED |
| Schmorl Technique | used to demonstrate reducing substances such as : melanin, argentaffin granules, formalin pigment ferric chloride-potassium ferricyanide produces turnbull blue precipitate mayer mucicarmine metanil yello |
| Schmorl Technique | reducing substances...........BLUE/GREEN goblet cell-mucin..............ROSE background.....................YELLOW-GREEN |
| Fontana Masson | demonstrates melanin and argentaffin granules argentaffin granules reduce silver naturally silver nitrate->gold chloride -> sodium thiosulfate -> nfr |
| Fontana Masson | Melanin.............................BLACK Argentaffin Granules..........BLACK Nuclei................................PINK |
| Rhodanine method | demonstrates copper detects copper especially in liver as in Wilson's disease rhodanine solution-> mayor hematoxilyn-> sodium borate |
| Rhodanine method | copper.......BRIGHT RED/YELLOW nuclei.........LIGHT BLUE |
| Gomori Methenamine Silver | demonstrates urates as found in gout deparaffinize and rinse with absolute alcohol->methenamine silver->sodium thiosulfate->light green counter |
| Gomori Methenamine Silver | urates.................BLACK background........BLUE/GREEN |
| Hall Stain for Bile | demonstrates bilirubin and can distinguish bile pigment from other pgments green color develops when the bilirubin oxidizes with ferric chloride in trichloroacetic acid medium called FOUCHET REAGENT *counter with van giesen (acid fuchsin/picric acid) |
| Halll Stain for Bile | bilirubin................GREEN background...........YELLOW |
Created by:
burkan2
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