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special stains

Minerals and Pigments

TermDefinition
Ferric Iron Fe 3+ Prussian Blue (equal parts potassium ferrocyanide and Hydrochloric acid) naturally found in small amounts in the bone marrow and spleen
Ferrous Iron Fe 2+ Turnbull Blue (potassium Ferricyanide w/ hydrochloric acid .4g to 40ml of .06N solution) used in the schmorl technique
Prussian Blue for ferric iron equal parts potassium ferocyanide and hydrochloric acid counterstain with nuclear fast red
Prussian Blue Nuclei & Hemofuchsin.....BRIGHT RED Hemosiderin (iron)....... BLUE Background...................PINK
Turnbull Blue for ferrous iron ferrocyanide solution wash in acetic acid
Turnbull Blue Ferrous Iron...... BLUE background......PINK/RED
Churukian-Schenk method for argyrophil granules demonstrates argyrophil granules in neurosecretory tumors
Churukian-Schenk argyrophil and argentaffin....BLACK nuclei......................RED background................YELLOW/BROWN
Grimelius for Argyrophil granules in neurosecretory tumors hydroquinone and sodium sulfite used to reduce silver nitrate solution
Von Kossa stain for calcium Silver reacts with the anions (carbonate and phosphate) of calcium salts a bright light is used to reduce the silver
Von Kossa calcium salts........BLACK background.........RED
Allzarin Red S stain for calcium place in allzarin red s solution and check progress microscopically
Allzarin Red S calcium deposits...ORANGE/RED
Schmorl Technique used to demonstrate reducing substances such as : melanin, argentaffin granules, formalin pigment ferric chloride-potassium ferricyanide produces turnbull blue precipitate mayer mucicarmine metanil yello
Schmorl Technique reducing substances...........BLUE/GREEN goblet cell-mucin..............ROSE background.....................YELLOW-GREEN
Fontana Masson demonstrates melanin and argentaffin granules argentaffin granules reduce silver naturally silver nitrate->gold chloride -> sodium thiosulfate -> nfr
Fontana Masson Melanin.............................BLACK Argentaffin Granules..........BLACK Nuclei................................PINK
Rhodanine method demonstrates copper detects copper especially in liver as in Wilson's disease rhodanine solution-> mayor hematoxilyn-> sodium borate
Rhodanine method copper.......BRIGHT RED/YELLOW nuclei.........LIGHT BLUE
Gomori Methenamine Silver demonstrates urates as found in gout deparaffinize and rinse with absolute alcohol->methenamine silver->sodium thiosulfate->light green counter
Gomori Methenamine Silver urates.................BLACK background........BLUE/GREEN
Hall Stain for Bile demonstrates bilirubin and can distinguish bile pigment from other pgments green color develops when the bilirubin oxidizes with ferric chloride in trichloroacetic acid medium called FOUCHET REAGENT *counter with van giesen (acid fuchsin/picric acid)
Halll Stain for Bile bilirubin................GREEN background...........YELLOW
Created by: burkan2