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Quiz 6 Oct-17
Osmotic Pressure
| Question | Answers |
|---|---|
| Do all microorganisms need water? | ~ Yes |
| Bacteria that can tolerate high salt concentrations is called: | ~ Osmotolerant |
| What is the name of the microorganism that needs high salt levels to grow? | ~ Halophiles |
| What 2 common things are used as food preservatives in high levels? | ~ Sugars and Salts |
| When something is equal in solutes it is called: | ~ Isotonic |
| When something has more solutes than another area it is called: | ~ hypertonic |
| When something has less solutes than an area it is called: | ~ hypotonic |
| What does "Obligate Aerobes" mean? | ~ needs oxygen |
| What does "Obligate Anaerobes" mean? | ~ cannot function in the pressence of oxygen |
| What does "Facultative Anaerobes" mean? | ~ grow better with oxygen, but can grow without |
| When something only needs small amounts of oxygen to live it is called: | ~ microaerophiles |
| Aerotolerant anaerobes means: | ~ indifferent to oxygen |
| Do all organisms produce superoxide (O2-)? | ~ yes |
| Is superoxide good for cells? | ~ No, it's toxic by stealing electrons |
| What is a type of Superoxide Dismutase? | ~ Hydrogen Peroxide |
| Hydrogen Peroxide is toxic to cells and it must be: | ~ Neutralized |
| Obligate Anaerobes lack: | ~ Superoxide Dismutase (SOD), and Catalase |
| Name some major elements that can conrol microbial growth? | ~ Carbon, Oxygen, Hydrogen, Nitrogen, Calcium, and Iron |
| How can trace elements help in microbial growth? | ~ by enzyme functions |
| Name 3 trace elements? | ~ Zinc, Copper, and Cobalt |
| When cultivating bacteria, name 4 media we might use? | ~ Complex, Chemically Defined, Selective, and Differential |
| Which media inhibits growth of many organisms, other than the one being sought? | ~ Selective |
| Which media shows a bacteria change a certain ingrediant showing a response? | ~ Differential |
| Which media is made of peptones and extract? | ~ Complex Media |
| Which media is made of a percise mixture of chemicals? | ~ Chemically Defined |
| Name two examples of a selective media? | ~ Brilliant Green Agar, and (EMB) Eosin Methylene Blue |
| What is an example of a differential media? | ~ Blood Agar Plates |
| Name 2 examples of Selective and Differential medias? | ~ Mannitol Salt Agar, MacConkey's Agar |
| What does the word enteric mean? | ~ Intestinal |
| Many Gram (-) enteric non-pathogenic bacteria can ferment: | ~ lactose, (salmonella cannot) |
| Name 4 methods to detect and measure bacterial growth? | ~ Direct cell counts, Viable cell counts, Measureing Biomass, and Measureing cell products |
| What is the difference between direct cell counting and viable cell counting? | ~ Viable is qualifying a number of cells capable of multiplying and direct is physical counting of each. |
| What are 2 cell counting instruments used with direct cell counting? | ~ Coulter counter and flow cytometers |
| Which method of measuring bacterial growth is considered an "indirect" method? | ~ Measuring Biomass |
| What are 3 products of a cell that might be used to measure? | ~ Acid, gases, and ATP |
| What are the 5 stages of bacterial growth in growth curve? | ~ Lag phase, log phase, stationary phase, death phase, and phase of prolonged decline |
| What phase does bacteria synthesize macromolecules in preparation for multiplying? | ~ the Lag phase |
| What phase does rapid growth (exponential growth) take place? | ~ the Log phase |
Created by:
Patrick24g
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