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Stains micro
| Question | Answer |
|---|---|
| Distinguishes gram-positive from gram-negative | Gran Stain |
| The primary stain in a Gram Stain | Crystal Violet |
| In a Gram stain, binds the primary stain to the cell walls of the gram-positive bacteria | Gram's Iodine |
| In a Gram stain, removes the primary stain from the gram-negative bacteria | Acetone-Alcohol decolorizer |
| The counter-stain in a Gran stain | Safranin |
| Amount of time that Crystal Violet is left on for | 2 minutes |
| Amount of time that Gram's iodine is left on for | 1 minute |
| Amount of time that safranin is left on for | 1 minute* |
| Color of Gram-positive bacteria after staining procedure is complete | Purple/violet |
| Color of Gram-negative bacteria after staining procedure is complete | Pink |
| used to stain members of the genus Nocardia | Kinyoun Stain |
| In the Kinyous stain, partial acid-fast Nocardia species retain carbolfuchsin stain and stain... | Red |
| In the Kinyoun stain, the 'negative' result would appear as bacteria that are the color... | Blue |
| The primary stain in the Kinyoun staining method | Carbolfushin |
| In the Kinyoun stain, this facilitates penetration of carbolfuchsin stain through the cell wall | Tergitol |
| The decolorizer in the Kinyoun stain | Acid aqueous solution |
| The counterstain in the Kinyoun stain | Methylene blue |
| Used to stain members of the genus Mycobacterium | Ziehl-Neelsen stain |
| What color will Mycobacterium be in a Ziehl-Neelsen stain? | Red |
| The 'negative' result for a Ziehl-Neelsen stain is the color... | Blue |
| The primary stain in a Ziehl-Neelsen stain | Carbolfushin |
| Helps the primary stain penetrate the lipid-rich cell wall in a Ziehl-Neelsen stain | Heat |
| Is the decolorizer in a Ziehl-Neelsen stain | Acidic alcohol solution |
| The counterstain in a Ziehl-Neelsen stain | Methylene blue |