Save
Upgrade to remove ads
Busy. Please wait.
Log in with Clever
or
show password
Forgot Password?
Don't have an account?  Sign up 
Sign up using Clever
or
Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account. Your email address is only used to allow you to reset your password. See our Privacy Policy and Terms of Service.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
focusNode
Didn't know it?
click below
 
Knew it?
click below
Don't Know
Remaining cards (0)
Know
0:00
edit
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

C11) Cell culturing

Cells and tissue culturing

QuestionAnswer
Define sterile conditions. Conditions that do not contain any living micro-organisms.
Define in vitro. When a particular procedure is used within a laboratory or equipment.
Define in vivo. When a particular procedure is used within a living organism.
Define clone. A copy of a molecule, cell, or organism.
Define tissue culture. When identical cells are grown in vitro to form a tissue.
Define bioethics. The study and discussion of what is good and bad about certain biological techniques.
Define generation time. The time for one complete generation, normally measured between successive births.
Define micropropagation. Techniques by which very tiny fragments of plants can be grown or propagated.
Define explant. A small piece, cut from a stock plant for the purpose of propagation, usually a section of a root, stem, or bud.
Define differentiated. A cell which a has developed a particular form or structure in order to fulfil a certain function in an organism.
Define propagate. To develop new individuals usually by asexual means in plants.
What are the steps of cell culturing? Tissue of interest is dissected to expose cells of the same type. Proteolytic enzymes are added to tissue to digest intercellular matrix of connective tissue and release cells. Cells placed in culture medium with sugars, salts and sometimes antibiotics.
How is the cell culture kept? In a flat flask at the optimum temperature (37 degrees for humans. Cells will usually grow quickly to cover the bottom of the flask.
Define the Hayflick Limit. Usually cultured cells will only grow for 30 to 40 generations.
Describe the technique for culturing plant cells. Explants of a sterile stockplant are removed. They are transferred to solution to be sterilised (remove microbes from surface) Transferred to culture vessel containing nutrient rich agar jelly and hormones and optimum conditions. FINISH*
Created by: MahaliaMcD
Popular Biology sets

 

 



Voices

Use these flashcards to help memorize information. Look at the large card and try to recall what is on the other side. Then click the card to flip it. If you knew the answer, click the green Know box. Otherwise, click the red Don't know box.

When you've placed seven or more cards in the Don't know box, click "retry" to try those cards again.

If you've accidentally put the card in the wrong box, just click on the card to take it out of the box.

You can also use your keyboard to move the cards as follows:

If you are logged in to your account, this website will remember which cards you know and don't know so that they are in the same box the next time you log in.

When you need a break, try one of the other activities listed below the flashcards like Matching, Snowman, or Hungry Bug. Although it may feel like you're playing a game, your brain is still making more connections with the information to help you out.

To see how well you know the information, try the Quiz or Test activity.

Pass complete!
"Know" box contains:
Time elapsed:
Retries:
restart all cards