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Exercise 3

Microscopy and Eukaryotic Cell structure

AB
Compound microscope instrument magnifies objects too small to be examined with the naked eye by using a system of lenses to enlarge the image up to 1,000 times
Substage diaphragm and condenser Unit these help regulate the amount of light passing through the specimen and the contrast in the image
Brightness regulator the rheostate should never be turned to maximum brightness. If your field of view is too dark, try adjusting the substage diaphragm FIRST and then increase the brightness regulator slowly.
The refocus the image use... the fine-focus knob only
Microscope Parfocal if the image is in sharp focus at low power, it will remain in focus when you switch to medium power
To increase the light intensity must first - adjust the substage diaphragm
At medium-power the depth of field is Shallower
Total Magnification= Ocular power(10x) X Objective Power
Total Magnifications possible 40x 100x 1000x
100X oil immersion
PLP magnifying power of low-power objective (10)
PMP magnifying power of medium-power objective (40)
DLP diameter of low-power field of view (micrometers)
DMP diameter of medium-power field of view (micrometers)
stage micrometer microscopists use to measure the field of view of a research microscope
Dissecting Microscope allow you to (1) observe specimens that are too large to be viewed with a compound microscope, and (2) observe specimens in 3 dimensions -light source is external
Stereoscopic dissecting microscope specimens shown in 3 dimensions
What does it mean by the light source being external? the light striking the specimen reflects off of it rather than passing around and through it as with a compound microscope
Created by: Jahazania
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