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M17)DNA Sequencing

Human beings can sequence even small amounts of DNA

QuestionAnswer
Describe DNA fingerprinting. DNA is extracted, cut using restriction enzymes, the fragments are sorted into bands by gel electrophoresis, DNA probes are added, and an x-ray is taken of the pattern.
Describe DNA polymerase. The enzyme that catalyses the reaction of DNA replication.
Describe DNA sequencing. A technique used to work out the actual sequence of DNA bases along a segment of DNA.
Describe electrophoresis. The separation of DNA segments in an electric field based on size and charge.
Describe polymerase chain reaction. A reaction in which millions of copies of the DNA can be made in a short period of time.
Describe radioactive probes. A radioactively labelled segment of DNA complementary to a desired gene.
Describe restriction segments. Segments of DNA that have been cut using restriction enzymes and separated using electrophoresis.
Describe auto radiography. A technique where DNA fragments, that have been labelled with radioactive elements and separated using electrophoresis, are visible on film sensitive to radiation.
What is the difference between DNA fingerprinting and DNA sequencing? DNA fingerprinting is a process in which the variable sections of DNA of an individual are subjected to electrophoresis to produce an autoradiograph of fragments of DNA whereas DNA sequencing works out the actual sequence of bases in a segment of DNA.
Which method which you use to analyse the DNA of a suspected criminal's hair follicle cells found at a crime scene? DNA fingerprinting
What method would you use to compare human DNA with chimpanzee DNA looking for differences in coding for a particular protein? DNA sequencing
What method would you use to analyse the DNA in an embryo looking for susceptibility to particular diseases? DNA fingerprinting
What are 2 situations where scientists might use the DNA PCR? In forensic science- From traces of blood, DNA extracted from the white blood cells, needs to be multiplies so that sufficient for DNA sequencing. Comparing DNA between species- DNA needs to be multiplied so that enough is available for DNA sequencing.
Created by: MahaliaMcD
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