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Biology Lab

Introduction

WordDefinition
What is a Theory? - brings together observations, laws, hypotheses and inferences into a logical explanation that describes natural processes - not random - not unsupported by evidence - not a guess - not unchangeable - must be constantly challenged and improved
Function of a Theory - change the way we perceive the universe - describe things that cannot be seen or perceived by our senses (does not deny their validity) - may be used to make predictions that help us with our lives
Predictions - inferences
Inferences a conclusion one draws by looking at the available observable data
Formulation of a Theory Experimentation, Observation, Recording, Analysis of Data
Basis for development of the embryo - cell growth - cell death - movement *these processes are also seen in the immune system and other aspects of the life of an organism.
Causes of cancer? - Deregulation of cell growth, differentiation and movement
DNA (deoxyribonucleic acid) - found in the nucleus - contains the genetic information that directs the cell - consists of genes
What is DNA made up of? - sugars - phosphate - bases
What bases make up DNA? - adenine - guanine - cytosine - thymine
What is a Triplet? groups of 3 bases - make up the code for one amino acid
Genome the sum of the entire DNA that carries information required for the survival of the organism
How is the information contained in DNA used to direct the cell? - DNA is transcribed to produce mRNA - mRNA is transported to the cytoplasm and is - translated by the ribosome to produce protein by a process known as protein synthesis
Involvement of Protein? - Maintaining structure of the cell - cell division - movement - enzymatic activity (conjugation of proteins)
What does protein actin do? gives the cell its shape and form
What does Adhesion molecules do? attach cells to surrounding tissue
What do Receptor proteins do? bind to hormones and cytokines on the surface of the cell
What do Hormones do? control the expression of genes within the cell
What do enzymes do? - are usually proteins (ex. trypsin- found in your stomach and intestines) - involved in digestion as well as DNA replication and protein synthesis
What's another thing that Proteins control the transcription of certain genes within the nucleus
Protein Synthesis requires an organelle for the translation of the mRNA in order to produce protein
Ribosome - an organelle - often found bound to membranes within the cell known as the endoplasmic reticulum= RER
How do ribosomes produce protein? The ribosome uses transfer RNA to bring different amino acids to the ribosome and bind to the mRNA to produce a protein with the correct sequence of amino acids
How are different proteins produced? By different combinations of amino acids which gives rise to different proteins with different properties
Mitochondria - an organelle - produces energy that is required for the processes of transcription, protein synthesis and DNA replication to occur - where the Krebb's Cycle is involved
Chromosomes - 46 chromosomes in each human cell - contains instructions for the production of protein - made up of DNA
Cell Division - how the body grows - cell first doubles then divides - involves a process called Mitosis - entire DNA has to be faithfully copied and equally divided between the two daughter cells - organelles must also be separated equally
Causes of Mutations - mistake in copying DNA - Cosmic radiation - Carcinogens as cigarette smoke and overdone burgers
If Mutations aren't corrected? - passed onto daughter cells - but if present in germ cells will be passed on to offspring
Why is a cell either a hair cell or a liver cell? - because all cells don't express the same genes - different cells have different proteins in them
What happens when a cell does not express the protein its supposed to? Like developmental defects as Achondroplasia (form of dwarfism)? - caused by a change in the amino acid sequence of a single protein in the cell - leads to substitution of the amino acid Glycine with Arginine - gene encoding this protein= chromosome 4
What does a change in a single amino acid sequence provoke? a single change leads to a protein that cannot function properly in the production of long bones
Alleles different versions of the same gene
Different alleles different versions of a protein
Down Syndrome - major defect in DNA replication and Mitosis - presence of an extra copy of Chromosome 21
Positive about Mutations - can help organisms adapt to changes in the environment - driving forces of Evolution
Biotechnology - ability to manipulate DNA - cut and ligate= create new combinations of genes= modify organisms
Examples of manipulating DNA - genetically modified plants - resistant to insects - genetic content can be modified using Plasmids
Plasmids circular pieces of DNA
Gene transfer move genes from one organism to another
Gene transfer principle: Genetic code is universal - gene from one organism can be expressed in an unrelated organism
Agrobacterium tumefaciens - causes tumors in plants - can be modified and remove the gene that brings good benefits like toxin for insects
Bacterial transformation process in which the modified plasmid is reintroduced into bacteria
Created by: Jahazania
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