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Biology Lab
Introduction
| Word | Definition |
|---|---|
| What is a Theory? | - brings together observations, laws, hypotheses and inferences into a logical explanation that describes natural processes - not random - not unsupported by evidence - not a guess - not unchangeable - must be constantly challenged and improved |
| Function of a Theory | - change the way we perceive the universe - describe things that cannot be seen or perceived by our senses (does not deny their validity) - may be used to make predictions that help us with our lives |
| Predictions | - inferences |
| Inferences | a conclusion one draws by looking at the available observable data |
| Formulation of a Theory | Experimentation, Observation, Recording, Analysis of Data |
| Basis for development of the embryo | - cell growth - cell death - movement *these processes are also seen in the immune system and other aspects of the life of an organism. |
| Causes of cancer? | - Deregulation of cell growth, differentiation and movement |
| DNA (deoxyribonucleic acid) | - found in the nucleus - contains the genetic information that directs the cell - consists of genes |
| What is DNA made up of? | - sugars - phosphate - bases |
| What bases make up DNA? | - adenine - guanine - cytosine - thymine |
| What is a Triplet? | groups of 3 bases - make up the code for one amino acid |
| Genome | the sum of the entire DNA that carries information required for the survival of the organism |
| How is the information contained in DNA used to direct the cell? | - DNA is transcribed to produce mRNA - mRNA is transported to the cytoplasm and is - translated by the ribosome to produce protein by a process known as protein synthesis |
| Involvement of Protein? | - Maintaining structure of the cell - cell division - movement - enzymatic activity (conjugation of proteins) |
| What does protein actin do? | gives the cell its shape and form |
| What does Adhesion molecules do? | attach cells to surrounding tissue |
| What do Receptor proteins do? | bind to hormones and cytokines on the surface of the cell |
| What do Hormones do? | control the expression of genes within the cell |
| What do enzymes do? | - are usually proteins (ex. trypsin- found in your stomach and intestines) - involved in digestion as well as DNA replication and protein synthesis |
| What's another thing that Proteins control | the transcription of certain genes within the nucleus |
| Protein Synthesis | requires an organelle for the translation of the mRNA in order to produce protein |
| Ribosome | - an organelle - often found bound to membranes within the cell known as the endoplasmic reticulum= RER |
| How do ribosomes produce protein? | The ribosome uses transfer RNA to bring different amino acids to the ribosome and bind to the mRNA to produce a protein with the correct sequence of amino acids |
| How are different proteins produced? | By different combinations of amino acids which gives rise to different proteins with different properties |
| Mitochondria | - an organelle - produces energy that is required for the processes of transcription, protein synthesis and DNA replication to occur - where the Krebb's Cycle is involved |
| Chromosomes | - 46 chromosomes in each human cell - contains instructions for the production of protein - made up of DNA |
| Cell Division | - how the body grows - cell first doubles then divides - involves a process called Mitosis - entire DNA has to be faithfully copied and equally divided between the two daughter cells - organelles must also be separated equally |
| Causes of Mutations | - mistake in copying DNA - Cosmic radiation - Carcinogens as cigarette smoke and overdone burgers |
| If Mutations aren't corrected? | - passed onto daughter cells - but if present in germ cells will be passed on to offspring |
| Why is a cell either a hair cell or a liver cell? | - because all cells don't express the same genes - different cells have different proteins in them |
| What happens when a cell does not express the protein its supposed to? Like developmental defects as Achondroplasia (form of dwarfism)? | - caused by a change in the amino acid sequence of a single protein in the cell - leads to substitution of the amino acid Glycine with Arginine - gene encoding this protein= chromosome 4 |
| What does a change in a single amino acid sequence provoke? | a single change leads to a protein that cannot function properly in the production of long bones |
| Alleles | different versions of the same gene |
| Different alleles | different versions of a protein |
| Down Syndrome | - major defect in DNA replication and Mitosis - presence of an extra copy of Chromosome 21 |
| Positive about Mutations | - can help organisms adapt to changes in the environment - driving forces of Evolution |
| Biotechnology | - ability to manipulate DNA - cut and ligate= create new combinations of genes= modify organisms |
| Examples of manipulating DNA | - genetically modified plants - resistant to insects - genetic content can be modified using Plasmids |
| Plasmids | circular pieces of DNA |
| Gene transfer | move genes from one organism to another |
| Gene transfer principle: | Genetic code is universal - gene from one organism can be expressed in an unrelated organism |
| Agrobacterium tumefaciens | - causes tumors in plants - can be modified and remove the gene that brings good benefits like toxin for insects |
| Bacterial transformation | process in which the modified plasmid is reintroduced into bacteria |