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SCCC Microbiology
Study help for the lab practical
| Question | Answer |
|---|---|
| A distinct thick gelatinous material that surrounds some types of bacteria | Capsule |
| A type of dormant cell that is extraordinarily resistant to damaging conditions (heat, UV light, toxic chemicals, etc.) | Endospore |
| Bacteria that have a thin cell wall; cells are pink when stained | Gram-negative Bacteria |
| Bacteria that have a thick cell wall; cells are purple when stained | Gram-positive Bacteria |
| A tool which uses visible light for observing microorganisms | Light Microscope |
| Can magnify images in excess of 100,000x | Electron Microscope |
| A compound microscope is a modern light microscope that has what two types of magnifying lenses? | An Objective Lens and an Ocular Lens |
| Magnifies the image, usually 10-fold (10x) | Ocular Lens |
| Focuses the light on a modern light microscope | Condenser |
| The product of the magnifying power of the ocular lens and the objective lens | Total Magnification |
| The bending of light rays | Refraction |
| Dyes that carry a positive charge | Basic Dyes |
| Dyes that carry a negative charge | Acidic Dyes |
| Used to detects members of the genus Mycobacterium; these organisms do not readily take up stains | Acid-fast Stain |
| Stains endospores; these dormant cells do not readily take up stains | Endospore Stain |
| Viscous capsules do not readily take up certain stains; the capsules stand out against a stained background | Capsule Stain |
| A substance that increases the affinity of cellular components for a dye | Mordant |
| An acidic dye is used to stain the background against which colorless cells can be seen | Negative Staining |
| Spherical bacteria | Coccus (pl: cocci) |
| Cylindrical bacteria | Bacillus (pl: bacilli) |
| Short curved rod | Vibrio (pl: vibrios) |
| Curved rod long enough to form spirals | Spirillum (pl: spirilla) |
| Cocci that typically occur in pairs | Diplococci |
| Type of media that enhances the isolation procedure by inhibiting growth of some organisms while encouraging the growth of others | Selective Media |
| Media that contain indicators to expose differences between organisms | Differential Media |
| A selective and differential medium developed to favor growth of Staphylococcus and to differentiate S. aureus from other members of the genus | Mannitol Salt Agar |
| A simple selective medium designed to inhibit Gram-negative organisms | Phenylethyl Alcohol Agar (PEA) |
| Selective for Gram-negative organisms, and both contain indicators to differentiate lactose fermenters from lactose nonfermenters; contains methylene blue | Eosin Methylene Blue (EMB) |
| A selective and differential medium containing lactose, bile salts, neutral red, and crystal violet; inhibits growth of Gram-positive bacteria | MacConkey Agar |
| An undefined, differential, and selective medium that allows growth of Gram-positive organisms and stops or inhibits growth of most Gram-negative organisms | Columbia CNA with 5% Sheep Blood Agar |
Created by:
capnkumo
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