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Immunology Lab
| Question | Answer |
|---|---|
| most abundant blood cell that functions to transport Oxygen | erythrocyte |
| blood cell responsible for blood clotting and blood vessel repair; second most abundant in blood | platelets |
| blood cell that functions to phagocytos, has 4-lobe nuclei and purple granules | neutrophils |
| blood cell with specific immunity; has large round nucleus | lymphocyte |
| largest blood cell that phagocytoses and presents Ag | monocyte |
| blood cell that destroys antibody-Ag complexes and fights parasites; has bright reddish-orange granules | eosinophils |
| least prevalent blood cell that may prevent clotting in inflammation | basophil |
| fluid portion of the blood in which all blood cells are suspended | plasma |
| if cells and clotting factors are removed by allowing the blood to clot, the yellowish fluid that remains is: | serum |
| removal of B-cells from a mixture by placing the cell mixture in a petri plate coated with anti-Ig Ab | panning |
| this can be produced by injecting an experimental animal with the desired antigen to stimulate humoral immunity | antiserum |
| this adjuvant contains Arlacel A (mannide monooleate-emulsifying agent), Bayol F (paraffin oil), and killed Myobacterium tuberculosis | Complete Freund's Adjuvant |
| This adjuvant lacks the Mycobacterium component, and is used for secondary booster immunizations because the Mycobacterium causes a severe hypersensitivity reaction if injected more than once | Incomplete Freund's Adjuvant |
| the aggregation of soluble antigen | precipitation |
| these reactions are more sensitive than precipitation and are caused by bridging of antigen particles by Ab molecules to form larger aggregates | agglutination |
| range of optimal concentration at which the greatst amount of precipitation occurs | equivalence zone |
| using boiling and reducing agent to break disulfide bonds | denaturing |
| pH buffer in tracking dye | Tris |
| increases density of sample; tracking dye component | glycerol |
| used in tracking dye to show how far it traveled | bromophenol blue |
| reducing agent in tracking dye | beta-mercaptoethanol |
| component of tracking dye that gives charge/mass ratio, keeps proteins denatured | SDS (sodium dodecyl sulfate) |
| "de-salting" | dialysis |
| simple and effective method of purifying biological substances that involves treating an insoluble support matrix such as agarose beads with chemicals which make the support material reactive to binding a ligand. | Affinity chromatography |
| this protein is used to purify IgG Ab from globulin salt cut | Protein A |
| common method used for obtaining information about purity and molecular weight of proteins | PAGE (polyacrylamide gel electrophoresis) |
| In SDS-PAGE, proteins move through the gel matrix because of what kind of charge? | negative |
| lower, larger layer of SDS-PAGE gel is called | separating gel |
| smaller, upper layer of SDS-PAGE gel is called | stacking gel, and is much lower in acrylamid concentration |
Created by:
charsany
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