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spore stain ex 12_ac
spore stain exc 12_ acid fast bacteria
| Question | Answer |
|---|---|
| Bacillus & Clostridium | 2 genera of medically significant bacteria that produce spores |
| endospore | structure first produced inside the cell |
| central spore | endospore in the middle of the rod |
| terminal spore | endospore at the end of the rod |
| subterminal spore | in between |
| spore | with time the cytoplasm around the endospore disintegrates, leaving a free spore |
| bacterial spore | dormant resistant stage, resistant to heat, drying, radiation, chemical disinfectants. Remain dormant(alive but not feeding) in dust for centuries |
| Bacillus subtilis | common labratory contaminant |
| what stain can not penetrate the spore wall | gram stain - it appears clear, unstained ovals shapes |
| what stain can penetrate the spores interior | ziehl-Neelsen acid fast will sometimes stain spores |
| schaeffer-fulton spore stain | hot malachite green penetrates the spore wall to dye the spores green. The stain is then cooled to trap it inside the spore. Any remaining cytoplasm outside the spore are stained red by the counterstain,safranin |
| B. anthracis | organism first used to prove the germ theory was a spore former |
| Robert Koch | proved the germ theory |
| Are spores involved in bacterial reproduction | yes, its called genetic recombination(parasexuality) transfer of genetic material from donor cell to recipient cell,there is no meiosis, formation of gametes and zygote |
| difference between spore and endospore | endospore - produced inside the cell, mechanism of survival not reproduction. spore - no detectable metabolism (cryobiotic), resistant to environmental stress high temp, strong acids, disinfectants |
| what bacteria uses the acid fast stain | mycobacterium & nocardia |
| m. tuberculosis | pathogenic to humans |
| What color and morphology do you expect Mycobacteria to be when observing an acid-fast stained slide on the oil immersion lens? | red stain and rod shaped clusters |
| Is Mycobacterium tuberculosis a fast or slow grower? | slow grower |
| 1.List two species of bacteria that are acid-fast and the disease each causes? | M. tuberculosis which is the causative agent of tuberculosis.M. leprae is the causative agent of leprosy |
Created by:
Jyen
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