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MCBL - Enzymes

Page 4

QuestionAnswer
Spectrophotometry it determines the light absorbance of a liquid or substance and measures enzyme activity.
Transmittance the ratio of intensity of the light transmitted by a material (It) to the intensity of the light striking the material (I0). T = It / I0
Absorbance the negative logarithm of the transmittance; Absorbance increases as concentration decreases A = log 1/ T
Beer-Lambert Law explains the direct relationship between the absorbance of a material and its concentration.
Enzymes Glucose oxidase and Peroxidase
Substrate reacting molecule of a chemical reaction
Product during chemical reaction substrate is converted to this
Active cite the region where the substrate binds to the enzyme
Enzyme-substrate complex the binding of the enzyme to its substrate
Cofactors inorganic ions such as cobalt, magnesium or zinc
Coenzymes organic molecules such as NAD or FAD
Competitive inhibitors mimic the substrate and compete for the active site, reducing enzyme activity
Non-competitive inhibitor binds to other areas on the enzyme and it alters the shape of the enzyme and its active site so it no longer can receive the substrate
Created by: aduchini
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