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micro lab exam 2

labs 9-13

QuestionAnswer
def of antigen; what is it usially made of a substance, usually a foreign protein that stimulates our immunity; a protein
def antibody a protein structure you make to help eliminate the antigen
antibody titre: this determines what; how is this done; what is the endpoint; what is the endpoint dilution called; how is the titre writtten the levels of antibody in the serum; by adding a constant amount of antigen and looking at the endpoint; the last tube that showed a positive reaction; the titre of antibodies; as a whole # (ex 640 is titre of dilution 1:640)
antibody titre: what pipette is used; what is process; how do youread the titre; what are the small particles; what is negative reaction 5 mL serp;pgic pipette; 0.5 ml is transferred from tube one to tube two, two to 3 and so on and this is how the antiserum is diluted out; gently tap the bottom of each tube if there are small particles that means it is a + result; agglutination; turbidity
controls: why are they used ; how do they pinpoint errors; to increase the reliability of your results; by using positive and negative response controls
epitope: aka; def; what binds to this antigenic determinant; the portin of an antigen that the immune system responds too; the antibody
def of sereoconversion Seroconversion is the development of detectable specific antibodies to microorganisms in the blood serum as a result of infection or immunization. Serology (the testing for antibodies) is used to determine antibody positivity. ...
antibodies: how many polypeptide chains are there; what connects the chains; what are the 2 arms that bind the antigen termed; what is the rest of the molecule called; what does Fc portion bind to; 4; disulfide bond; the antigen bindinf fragments (Fabs); crystallized fragment (Fc); various cells in immune system itself (diagram pg 461)a
def of agglutination; what does antibody-antigen linkage produce; def of particulate; can it be seen with the naked eye a visable mass of bacteria/ RBcs and antibodies bound to eachoutehr; a large cluster of cells cross linked; the antigens are large in size; yes
antibody titre: antibody levels in the blood reflects past what; def of serum; does serum contain Abs exposure to an antigenic determinant; the liquid portion of a blood sample from which RBCs, WBCs and paltelets have been removed; ys
Abs titre: lack of antibody titre could mean what; presence of abs from pt means __ has occured; no exposure to agent tested or exposed recently to agent and insufficient time has occured to produce antibodies; seroconversion;
antiserum contains what antibodies
titre: def of acute serum titre; def of convalescent serum titre; collected during early onset of CMs; collected weeks after exposurel
hepatitis titre: aka; what results mean nothing more needs to be done; when does pt have seroconverted anti-HBs; is adequate anti-Hbs are present; if they developed antibodies
slide agglutination technique: what is done; what side is control; a drop of proteus antiserum is placed on the left side of a slide and the physiologic saline is placed on right side of slide then culture is placed on right and left side and the side is rocked back and forth ; right side;
parcipitation reaction: def; what is produced during many infections; what diffuse towards eachother; what cross links to form a lattice; def of lattice; what can this test identify; serological reactions involviing small soluble atnigens and antibodies produced against them ; precipitatiing antibodies; antigen and specific antibodies for that antigen; antigens and antibodies; a visible precipitation reaction; antigen or antibody
precipitate reaction: when the lattice is very large what can be observed; def of zone of equivalence; visible particles; the concentration of the antigens and antibodies are equivalent and a lattice is formed and the particles can be seen;
fluid precipitation: how is it done; when can the zone of equilenance be reached; antigens are suspended in fluid and combined with antibodies suspended in fluid, they are not mixed and are allowed to diffuse towards eachother; if the antigen is capable of binding with the antibody;
gel precipitation: what medium is used; what is done to the agar; what is placed inthe 3 outside holes; what is placed in the middle hole; what happens; a semisolid medium agarose plate; ; 4 holes are bored into it; antigen; unknown antibody; the antibodies and antigens diffuse towards eachotehr producing a band of parcipitate
gel precipitation: what is purpose of this test; when do the antibodies and antigens stop diffusing towards eachother; what forms when the zone is reache;d why does the media have to be semisolid; allows for determination of an unknown antibody; when a zone of equilalence is reached; an opaque band of precipitate forms between the well; it allows for diffusion;
latex agglutination test: what is coated with a known antigen; what is mixed with known antigen latex suspension; what does it mean if agglutination is positive; latex beads; patient serum and controls; patient has produced antibodies against antigenic determinant of the antigen
ELISA: aka;purpose; what diseases are screened; what hormone test uses this; enzyme linked immunosorbent assay; used to detect the presence and amount of either antigen or antibody in a sample; HIV, rubellal; pregnancy test;
ELISA: why used; what does the direct one detect; what does the indirect one test; to detect antigen in a sample of antibody in a sample; the presence of antigens in a saomple; the presence of antibodies in a sample;
ELISA: why are the wells washed after each step; why are tests run in triplets; to remove unbound/ nonreactive antibodies; for accuracy;
what is an example of a commercial agglutination test monospot test
infectious mononucleosis: etiologic agent;what is burkitts lymphoma; why is at risk for this lymphoma; epstein baarvirus; a b-cell malignancy that develops in jaw and swells in cheeks; african children 4-8 yo b/c of weakened immune systems
infectious mononucleosis: how is it spread; pathogenesis; incubation; s/s; does immunity develop; recently infected can shed virus for how long; kissing, oral contact, saliva and blood transfustions; the virus infects human B lymphocytes and enters through the oropharynx and invades; 30-60days; sore throat, HA, fatigue, general malaise; yes; 18mo
def of antitoxin antibody w/ ability to neutralize specific toxin
def of adjuvant any compound that enhances immunogenicity and prolongs antigen retention at the injection site;
def of monoclonal antibodies pure antibodies
natural killer cells: are they nonspecific or specific; what do they do; nonspecific; surkey cells looking for ones missing MHC 1 receptor;
Dx hepatitis B: why is an acute sample take; why is a convalescent sample taken; acute is done early in disease and should have titre of 40; thisis done later in disease, titre is 160 to show that disease is in process
disinfection: def; examples; the destruction or removal of vegetative pathogens but not bacterial endospores on inanimate objects; boiling water, pasteurizaion, UV light, liquid chemicals;
sterlization; def; examples; the complete removal or destruction of all viable microbes, used on inanimate objects; incineration, dry ovan, steam autoclave, xrays, chem gases, chem liquids;
antisepsis: def; examples; chemicals applied to body surfaces to destroy or inhibit vegatiative pathogens; chem liquids;
decontamination: def; example; the machanical removal of most microbes; air and liquids
gram positive bacteria: examples; what of the two are spore formers; what layer is thick; what links peptidoglycan molecules; does it have surface proteins; bacillus sp. and staphylococcus aureus; bacillus; peptidoglycan layer of cell wall; teichoic acid; yes;
gram negative bacteria: examples; are any spore formers; what one is a strick aerobe; what layer is thin; what else does it have that is part of cells wall; name the layers f=of outer membrane; E. Coli, pseudomonas sp.; no; pseudomonas sp; peptidoglycan layer; outer membrane; external layer and inner layer
what is the most common agent used to kill microbes inthe lab autoclave
the effects of heat: what is the moist heat needed to kill bacterial endospores; how does moist heat kill microbes; what type of microbes are most resistant to heat; 120 C; by altering the 3D form of proteins and this destroys the activity of enzymes and the componets of structural proteins; spore formers
effects of heat: if there is clear tube with no growth in the tube what can be determined; the microbe were destroyed by heat;
bacterial endospores: is it a reporductive structure; what two genera can form endospores; what is it highly resistant to; when environment is favorable, what does spore germinate into; no; bacillus sp. and clostridium sp,; heat, chemicals and changes in pH; a single vegatative cell;
UV light:is it invisable to our eyes; how is it abosrbed; how many reactions does each cell of skin experience every second; yes; by a double bond in pyrimidine bases and opening the bond allows it to react with neighboring molecules50-100;
UV light: when are cells unable to repair the damage; what wavelengths can cause most damge; when the duration of exposure to UV light is sufficient they can't keep up with the rate of DNA modification; short (germicide);
UV light: short wavelengths are used how; why is DNA unable to replicate during binary fission; to prevent microbial growth on work surfaces in ittus culture hoods; b/c the light alters the bases;
def antiseptic used when destroying microbes on tissue;
def of static if a substance inhiits growth of a microbe
what virus is very resistant to substances Hep A
chemical antimicrobials: def cidex; def of betadine; def lysol; an aldehyde/alkalating agent; iodine compound and a halagon; alphenol comound;
antimicrobial agents in food: how is this test done; how is zone measured; NA plate is inoculates with bacteria and discs soaked in food is placed on the plate and zone of inhibition is measured; in mm;
zone of inhibition: what to measure if there is no zone; if the bacteria grows to the disc what does this mean; if there is any inhibition beyond the disc diameter what does this mean; the bigger the zone means what measure the size of disc; the bacteria are resistant to the chemicals; the the organism is susceptible; the more inhibitory the substance
egg whites; what enzyme do they contain; lysozymes are effective against what; lysozymes; gram + bacteria
kirby bauer susceptibility test: this test determines what; what are the results used for;the density of inoculum must be standard to what; the susceptibility of bacteria to antimicrobial agents; to determine the correct antimicrobial agent to be prescribed to pt;mcfarland standard;
kirby bauer susceptibility test: waht agar is used; MH agar is how thick; mh agar has what pH; what grows on it; how long incubated; what is measured; how is agar inoculated mueller hinton;4 mm; neutral; all but fastidious bacteria; 18-24 hr; zone of inhibition; lawn tech
kirby bauer susceptibility test: def susceptible; def intermediate; resistant the organism is killed or inhibited by the antimicrobial concentration; organism may not be killed or inhibited by antimicrobial concentration; organism not kiled
microbial control methods: ex of physical agents; ex of chemcial agents; ex of mechanical agents heat (dry, moist), or radiation; gases, liquids; filtration (air or liquids)
variables: what is an expiramental varaible; what is control variable; the thing that changes (types of disinfectants); the control stays the same (microbe used)
def of penicillinase an enzyme produced by penicillin resistant strains of bacteria to inactivate penicillin into a harmless chemical
def of mic value ; what does MIC use this provides the minimum dilution of an abx that would be effective in trating a specific bacterial infection; a dilution tray containging supported medium
what are the 4 things that need to be standard in the kirby bauer test; mueller hinton agar; standard abx discs, standard inculim, incubate for 18-24 hours at 37 degrees C
respiratory pathogens: streptococcus sp.: where do they grow best; what are O2 requirements; what are CO2 requirements; how do we get the atmospheric requirements; blood agar; reduced; increased; using a candle jar and CO2 incubator;
respiratory pathogens: streptococcus sp.: what is the shape of the genus; what is gram stain; cocci; gram +;
def virulence factors are either structures or products that enhance pathogenicity
respiratory pathogens: streptococcus sp.: what is the most common hemolytic type; alpha hemolytic;
name some indigineous flora in the respiratory tract strep. sp, staph. sp., neisseria sp., corynebacterium sp., several strict anaerobes., candida albicans
respiratory pathogens: streptococcus sp.: what are the 3 hemolytic patterns that they can have; beta, gamma, alpha;
streptococcus sp.: beta hemolysis- def; what are the enzymes; what will the bacterial colonies look like on the blood agar; the bacteria's hemolytic enzymes lyses the blood cells in the agar plate; streptolysin O and S; clear around them;
streptococcus sp.: alpha hemolysis- def; what will the colonies look like; means that the bacterial enzymes only partially break down the blood cells; green to brown discoloration of the agar around the bacterial colonies;
streptococcus sp.: gamma hemolysis- def; whhat will colonies look like essentially no hemolysis at all, the bacterial do not produce enzymes that affect the RBCs; no change in color;
streptococcus sp.: beta hemolysis- if the organism shows beta hemolytic pattern what is next test done; what does positve strep quick test mean; what is done if strep quick is negative; strep quick test; the organism is S. pyogenes; set up bacitracin disc;
streptococcus sp.: beta hemolysis- what organism is it if bacitracin disc is sensitive; what is it if it is resistant; S. pyogenes; S. Agalactiae
what is group A strep; what is group B strep S. pyogenes, S. agalactiae
streptococcus sp.: alpha hemolysis- if organism shows this what test is done next; what organism is it if it is sensitive to optochin discs; what is it if it is resistant to disc optochin disc; S. pneumoniae; s. viridans
streptococcus pyogenes: what group of strep; what type of infection; what is sequelae if not treated; what do colonies look like; what hemolytic pattern; gram stain; shape; Dx A; painful and self limiting; rheumatic fever, glomerlonephritis, scarlet fever; small/med white entire margin; beta; +; streptococci; quick strep pos, and bacitracin sensitive
streptococcus pyogenes: tx; what is tx for those allergic to penicillin; penicillin for 10d; erythromycin;
streptococcus pyogenes: sequelae-scarlet fever- what is tx; what is rash due to; exotoxin damages what abx; a particular strain of the bacterium that produces an erythrogenic exotoxin; capillaries;
streptococcus pyogenes: sequelae- what two infections will abx not work on; what is damages in glomerulonephritis; whatis damaged in rheumatic fever glmerulonephritis and rheumatic fever; damage to nephrons; damage to joints, heart and other tissues
streptococcus pyogenes: sequelae- scarlet fever- who gets it; when is onset; what has rash; what s/s on tongue; when does rash fade; sx; children; < 12 hr; cheeks/ neck and thorax; strawberry red/white coating; about 6 day; S. pyogenes from oropharynx
LRT organisms: what are pathogens from URT and inhaled from outside of body
LRT pathogens: streptococcus pneumoniae- most common cause of what; potential cause of what; colonie appearance; what is hemolytic pattern; gram stain; shape; does it have a capsule; what disc is it sensitive to; bacterial pna; meningitis; medium, mucoid, white, entire margin; alpha; + , diplococci; yes; optochin;
LRT pathogens: streptococcus pneumoniae- tx; what is tx if there is a resistant strain; what does gram stain look like in sputum penicillin; fluoroquinolones and vancomycin; vaccine; gram+ diplococci with neutrophils
LRT pathogens: klebsiella pneumoniae- what tyep of pna; conoly charecteristics; gram stain; does it have a capsule; shape bacterial; medium/ large mucoid entire margin, off white to beige colonies; negative; yes; baiclli
bacitracin disc: a zone of inhibition means what; the test is positive and organism is susceptable and the organism is S. pyogenes;
optichin disc: what does a zone of nihibition more than 18 mm mean; test is positive and organism is S. pneumoniae
throat culture: any of the normal flora is considered ____ pathogens; opportunistic;
capsule stain: is this a virulence factor; ex of resp. tract organisms that have these; yes; S. pneumoniae and klebsiella sp.
acid fast stain: used for what disease and what organism; M. tuberculosis has what in its cell walls; this prevents what; what would false gram stain be; TB and mucobacterium tuberculosis; lipids; decolorizing when using acid alcohol; gram - (it is not this)
corynebacterium diphtheriae: agent of what disease; divide so fast that bacilli look like what; diphtheria; chinese like letters;
maemophilus influenzae- causes what disease in young children; is it fastidieous; it can couase secondary what; meningitis; yes; pna
what role does ___ play in protecting the respiratory tract against pathogens . . . ciliated epithelium, normal flora; alveolar macrophages; secretory IgA moves mucus with trapped organism and debris out of the respiratory tract; colonizes areas of body to prevent or decrease access ot the area by a pathogen; phagocytizes debris and organsims that reach alveoli; found in body secretions binds to microbes
neisseria sp.: doe isolation of it from pharynx mean that a person has gonorrheal pharyngitis; what is gram stain; what type of pathogen; how is it fastidious it could be or it could be just normal flora; neg diplococci; intracellular; tempt and humiditty sensitive
what agent causes pneumococcal pna; what is from reaction; what is virulence factors streptococcus pna; gram + diplococi; capsule, streptokinase, hemolysin;
klebsiella pna: how does one get it; cause; transmission; tx when host defenses are impaired (malnutrition ect); klensiella pna; inhaled droplets; amikacin/ cephalosporins;
primary atypical pna: cause; transmission; can it be gram stained; aka; why can't it be gram stained; tx mycoplasma pna; inhalation of contaminated droplets; no; walking pna; b/c bacteria lacks cell wall; tetracycline or erythromycin
psittacosis: cause; transmission; what type of parasite is it; chamydia psittaci; dust, parrot like bird feces; obligate intracellular parasite; tetracycline
enteric bacteria: def; ex; where are the located; are they usually pathogenic or non pathogenic gram negative rod bacteria that are facultative anaerobes; E. coli, Proteus sp, salmonella and shigella sp, klebsiella pna, enterobacter sp, serratia sp, ; part of the normal intestinal flora or involved in GI diease; non pathogenic
enteric bacteria: biochemical properties;what media is used; some can ferment lactose, produce hydrogen sulfide, hydrolize urea, liquefy geletin, decarboxylate amino acids; selective and differential;
enteric bacteria: what should pH be; what does the medium inhibit; what does it select for; contains what indicators; changes color in presence of what; when organisms ferment what is produced; acculumation of acids does what; what are the 2 agars used 7; gram + bacteria and fastiduous gram neg; enterics; pH; in presence of lactose fermentation; carbohydrate acid; lowers the pH of the medium and change the color of the pH stain ; MAC and HE
enteric bacteria: MAC agar: what is the pH indicator stain; what will color be of lactose fermenters; what is color of non lactose fermenters; why is crystal violet and bile added lactose and neutral red; pink; colorless to beige; inhibits gram pos. bacteria and fastidious gram neg;
enteric bacteria: HE agar- aka; what is the pH indicator stain; what is color of lactose fermenters; what is color of non lactose fermenters; why is bile and bromythmol blue added; what would prove H2S production hektoen enteric agar; yellow organge colony; colorless; inhibits fast. Gram neg bacteria and gram pos; black colonies
enteric bacteria: SS agar: aka; what should the color of them be; are all other organisms inhbited; why is reagents added; what will colonies look like if they produce h2s; salmonella-shigella agar; lactose, neutral red dye, bile, reagents; samonella and shigella sp.; no; colorless; for the most part; to detect h2s; black;
enteric bacteria: SS agar: what produces H2S salmonella or shigella; what species will be black; what species will be beige; lactose fermenters have what color salmonella; salmonella; shigella; pink
bile on an agar plate inhibits growth of what gram pos and fastidious gram neg bacteria;
enteric bacteria: what agar contains the largest amount of bile; what one contains the least amount of bile ss; mac;
enteric bacteria:media- what are the 2 dyes in mac; what is the inhibitory agent of these media; neutral red and crystal violet; bile;
feces cultures use what type of media selective and differential mac HE and SS
bacteriodes sp. and clostridium sp are common bacteria in the l intestines but why are they not enterics; although bacteriodes is gram -, it is strictly and anaerobe and clstridium is gram positive and is an anaerobe spore former
def of coliform; why are these counts used to test purity of water a gram neg nonspore forming bacillus, some are lactose fermenters that are found in human feces; prescence of this indicates fecal contamination
why are abx not used in tx of salmonella b/c they can increase the carrier rate where the individual can continue to shed the organism in their feces
S. auerus: what does colony look like; what is hemolytoc pattern; is it coagulas pos. or neg; is it catalase pos or neg; mannitol fermentation pos or neg; where is this a common pathogen; gram stain golden tan color, medium size, entire, raised; beta; positve; positve; positve; positive; wound; gram positve staphylococci;
S. epidermidis: colony description; coagulase pos or neg; catalase pos or neg; mannitol fermentation pos or neg; gram stain; what is hemolytic pattern; where is it normal flora; is it often a pathogen; off white, small med size,raised, entire margins; neg; pos; neg; gram pos staphylococci; gamma; skin; rarely as subacute endocarditis
catalase test: used to identify what two species; strep is pos or neg; staph is pos or neg strep and staph; neg; pos
what 2 tests are used to differentiate staph species from eachother coagulase and mannitol
clostridium sp: what diseases; gram stain; does it have spores; oxygen requirements; where is it found; is it a wound pathogen botulism, tetanus, food poisening, gas gangrene; gram + streptobaccilli; yes; strict anaerobe; soil; yes
how can anaerobes be cultured with thiocollate broth and anaerobic chamber
thioglycollate broth: what does sodium thoglycollate do; why is resazurin added to medium; what oxygen requirements does micrococuss sp have; C. sporogenes have; E. coli have; it reduces oxygen in the medium; turns red in presence of O2; aerobe; anaerobe; facultative aneaerobe
anaerobe chamber: what is it used for; what reacts with o2; what happens with hydrogen gas pack is activated by water; what is added; what are colors for indicator to grow strict anaeobic microbes; the hydrogen gas pack; pallasoim catalyst to use up the oxygen and produce water;anaerobic indicator; blue if aerobic and white if anaerobic
what is the most common cause of cystitis E. coli
E coli: is it enteric; oxygen requirements; yes; facultative anaerobe
urine culture: colony count is the # of colonies times ___; this = what; what number indicates a UTI 1000; the # of organisms per mL in the patient; > 100,000 organisms / mL
gonorrhea: etiologic agent; when are CMs; trasmission; cms in males; CMs in FM; complications in wm; neisseria gonorhoeae; 1-3 days; close intimate contact; urethritis, frequent urination, sterility; in cervix only, rectal pain and all s/s of males; arthritis, sterility, PID
gonorrhea: newborn s.s; tx eye infection; silver nitrate/ erythromycin eye drops
gonorrhea: reservior; tx; gram stain; what medium does it grow in; dx for males; dx for females; humans; cephalsporins only; fastidious gram neg diplococci; thayer martin medium; gram stained urethral discharged specimen; vaginal culture and grma stain;
thayer martin medium: is it selective; is it differential; what is added; what type of agar; why are abx added yes; yes; aBX; chocolate; they inhibit other organisms
syphilis: reservoir; transmission; how long is incubation; dx humans; close intimate contact, organism penetration through mucous, wounds; 2-6 weeks; ID spirochetes from chancre useing flourescence microscopy and aB testing;
syphilis: primary: s/s; how not does it last; painless chancre sore near oral or genital region; 4-6 weeks
syphilis: secondary- where is organism in body; s/s; what percent is cured; what % is latent; what % get it again; blood, lymph, tissues; rash, allopecia and lesions in mouth; 25%; 25%; 50%
syphilis: tertiary- s/s; where is it localized; lesions; CV system, joints, CNS
syphilis: congenital: disease crosses placenta when; s/s; after the 3rd or 4th month; anemic, nasal discharge, rash, saddle back nose, lesions later, still born
chlamydia sp: def C. psittaci; def C. pneumoniae; def. C. trachomatic; birds and numan zoonotic infection; human to human transmission; human to human transmission
C trachomatic: neonate s.s; s/s as STD; corneal scarring, tranmitted during vag delivery; cervix infected; pelvic inflammatory Dx and infertility
staph. sp are tolerant to what; salt
catalase test: what is done; what happens when oxygen gass is released; positve means what is produced slide used and divided hydrogen peroxide is dropped on each half and then organism dropped;bubbles occur and organism is positive; the enzyme catalase
coagulase test: differentiates between what; when the enzyme procoagulase is mixed with plasma what is it convereted to; coagulase does what to the plasma; what will be seen on the slide; the 2 speceis of staph; coagulase; clots it; clumps if positive;
mannitol fermentation: mannital salt medium is selective yes or no; differential yes or no; what % of salt does it have; when mannitol is fermental what happens; yes; yes; 7%; color changes from red to yellow b/c or the acid production;
what sp produces the most potent exotoxin known to humans; constridium botulinums
are clostridia gram + or neg +
spore stain: what two species form thick walled endospores; are spores resiliant to staining; what is applied to assist in stain penetration; what will take up primary stain; waht is primary stain; what is decolorized with secondary stain; whatis secondar clostridium and bacillus; yes; heat; spores and vegatatvie cells; malachite green; vegatative cells; safranin
anaerobic jar: how is oxygen eliminated; by chem reaction
what are the common sites cultured for gonorrhea vagina, urethra, rectum and oral cavity
lactobacillus sp: where is this normal flora; what does it produce that protects the vagin from pathogens and prevent the overgrowth od candida albicans; vagina; acids;
def keratitis inflam of cornea of the eye
def salpingitis inflam of fallopian tubes
def pelvic inflammatory disease infla of femal genital tract accompanied by fever and lower ab pain
def gonococcal pharyngitis trhaot infection caused by gonorrea
def gonococcal proctitis inflam of rectum or anus caused by gonnorrea
Created by: jmkettel
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