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Lecture017
MB351Exam3
| Question | Answer |
|---|---|
| What two types of phosphorylation is used to generate ATP for both anaerobic and aerobic respiration? | Substrate-level and oxidative |
| How much ATP molecules is produced per glucose in aerobic respiration? | 36 in eukaryotes and 38 in prokaryotes |
| How much ATP per glucose is produced in anaerobic respiration? | Variable..fewer than 38 but more than 2. |
| What type of phosphorylation is used to generate ATP in fermentation? | Only Substrate level |
| What is the final hydrogen (e-) acceptor in fermentation? | An organic molecule |
| Fermentation can grow in what sort of conditions? | aerobic or anaerobic |
| What are the three e- acceptor of Anaerobic respiration? | NO3-, SO4-, and CO32- |
| What is the most common e- acceptor in anaerobic resp.? | Nitrate (NO3-) |
| What can nitrate be reduced to? | NO, N2O and N2 (gaseous) |
| What is denitrification? | This is the process where NO, N2O, and N2 is lost to the environment; gaseous N2 is formed biologically (look at flowchart in ppt) |
| List the order that nitrogen is broken down. | Nitrate(NO3-), Nitrite(NO2-), Nitric oxide(NO), Nitrous oxide(N2O), and finally Dinitrogen(N2). |
| What breaks down Nitrate to Nitrite? | Nitrate reductase |
| What breaks down Nitrite to Nitric oxide? | Nitrite reductase |
| What breaks down Nitric oxide to Nitrous oxide? | Nitric oxide reductase |
| What breaks down Nitrous oxide to Dinitrogen? | Nitrous oxide reductase |
| What is the name of the denitrifying bacteria? | Pseudomonas stutzeri |
| What are some uses for denitrification? | To clean up waste!Human waste, auto waste, pig waste |
| Denitrification and sulfate-reduction are what type of processes? | Dissimilatory (respiratory)processes |
| Is the energy yield in anaerobic respiration lower or higher than from using O2? | Lower energy yield than from using O2 |
| What happens if there is lower energy yield in anaerobic resp.? | Lower biomass |
| (Shewanella oneidensis) Facultative anaerobes are able to use how many different electron acceptors? | 12 different e- acceptors |
| What is fermentation? | Release of energy from oxidation of organic molecules |
| Does fermentation require oxygen? | No. It does not require oxygen. |
| Does fermentation use the krebs cycle or ETC? | Neither, it does not need those processes. |
| What is the final e- acceptor of fermentation? | an organic metabolite (pyruvate or derivative) |
| What is fermentation used to regenerate? | It is used to regenerate NAD+ from NADH without O2. |
| What are the 2 types of fermentation? | Lactic acid and alcohol fermentation (watch video) |
| What is alcohol fermentation? | it produces ethanol and CO2 |
| What is lactic acid fermentation? | It produces lactic acid |
| What are the two types of lactic acid fermentation? | Homolactic fermentation and Heterolactic fermentation |
| What does homolactic fermentation produce? | it only produces lactic acid |
| What does heterolactic fermentation produce? | it produces lactic acid and other compounds |
| Name some end products of fermentation? | Lactic acid, ethanol & CO2, Propionic acid and acetic acid, succinic acid, acetic acid, formic acid, etc... |
| What are some products of ethanol fermentation? | Beer, wine, fuel |
| What is Saccharomyces cerevisiae? | Starch is unable to be fermented so the barley is germinated. The seedling produces amylases that break down starch into fermentable carbohydrates (maltose). |
| How is malted barley made? | After germination of barley, heat is used to stop sprouting and dry the grain. The malted barley is roasted to varying degrees to make dark beer. |
| Name some food products made from fermentation? | Cheese, vitamin C, sausages, yogurt, bread, vinegar |