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DIT molecularbio

DNA/RNA/proteins

QuestionAnswer
location, pathway and nitrogen source of CPS I mitochondria, urea cycle, ammonia
location, pathway and nitrogen source of CPS II cytosol, pyrimidine synthesis, glutamine
rate limiting step in purine synthesis glutamine-PRPP amidotransferase
rate limiting step in pyrimidine synthesis CPS II
sources of carbons in the formation of purines CO2, THF, glycine
sources of carbon in pyrimidine synthesis CO2, aspartate
what accounts for the positive charge of histones? Arg and Lys
what accounts for the negative charge of DNA? phosphate groups
inhibits ribonucleotide reductase hydroxyurea
inhibits dihydrofolate reductase methotrexate, trimethoprim
inhibits thymidylate synthase 5FU
inhibits inosine monophosphate dehydrogenase mycophenolate
inhibits PRPP synthetase 6 mercaptopurine
FTT, megaloblastic anemia that doesn't improve with B12 or folate orotic aciduria
how does UV light damage DNA creates a thymine dimer on the same strand of DNA
replicates lagging strand, synthesizes RNA primer (euk) DNA pol alpha
repairs DNA (euk) beta, epsilon DNA pol
replicates mitochondrial DNA (Euk) gamma DNA pol
replicates leading strand DNA (euk) delta DNA pol
DNA repair defect. hypersensitivy to UV light xeroderma pigmentosum
DNA repair defect. sensitivity to ionizing radiation, immunodeficiency, ataxia beginning at 1-2 yo ataxia-telangiectasia
DNA repair defect. hypersensitivty to sunlight, leukemia and lymphoma, onset at 25yo bloom's syndrome
inhibits 50s peptidyltransferase chloramphenicol and streptogranins
blocks 50s, blocking translocation macrolides, linezolid
binds 30s, preventing attachment of tRNA tetracycline
inhibits prokaryotic RNA polymerase rifampin
inhibits prokaryotic topoisomerase flouroquinolones
inhibits prokaryotic dihydrofolate reductase trimethoprim
3 ways cells break down proteins ubiquitin, lysosomes, ca dependent enzymes
catalyzes peptide bond formation during protein synthesis peptidyl transferase
what enzyme matches amino acids to tRNA aminoacyl tRNA synthestase
mRNA stop codons UAG, UAA< UGA
RNA pol in eukaryotes 1 rRNA, 2 mRNA, 3tRNA
what amino acid frequently has more coding sequences in the mRNA than are represented in the peptide that is created from that mRNA methionine
How is hnRNA processed before it leaves the nucleus 5' cap, poly-A tail, intron splice
What is the characteristic sequence of the promotor region? RNA pol, TFs, 25 TATA 75 CAAT
what does a mutation in the promotor sequence cause? decrease in the amount of gene transcribed
What enzyme is deficient in Lesch-Nyhan syndrome? HGPRT
What is the treatment for lesch-nyhan you can treat the gout with allopurinol
what structural motifs allow for proteins to bind to DNA? helix loop heliz, helix turn helix, zinc finger, leucine zipper
Created by: kayjames