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Microbiology #3

QuestionAnswer
The use of the suffix "ase" on a word denotes a(n) a. substrate b.product c. enzyme d. electron acceptor e. a, b c. enzyme
Enzymes speed up reactions by a. raising activation energy b. producing heat c. producing pH d. lowering activation energy e. a and c d. lowering activation energy
Enzymes act on ______ to produce _________ a. products, catabolites b. substrates, products c. products, substrates d. glucose, anabolites b. substrates, products
Enzymes act as b. catalysts
Coenzymes are derivatives of a. minerals b. proteins c. lipids d. vitamins d. vitamins
Cofactors are derivatives of minerals
A process common to all living organisms, aerobic and anaerobic is a. glycolysis b. fermentation c. the krebs cycle d. electron transport chain e. pyruvate oxidation a. glycolysis
Which is true of competitive inhibition? a. substrate and inhibitor both bind to the active site b. substrate and inhibitor both bind to the different sites c. PABA is a competitive inhibitor d. Sulfa drugs are competitive inhibitor e. A and D e A and D
Which is true about DNA replication? a. refers to the structure single-stranded RNA b. is synonymous with semiconservative c. refers to the opposite orientation of the two strands in DNA d. refers to a type of prokaryotic replication c. refers to the opposite orientation of the two strands in DNA
Which would have the least effect on the amino acid sequence d. addition/deletion of 3 consecutive nucleotides
Which is not true of RNA? a. it is usually single-stranded b. it contains uracil c. it contains ribose d. there are 4 functional types e. none of the above d. there are 4 functional types
Which is true about the RNA transcript? a. it is formed using the DNA minus strand as a template b. it has the sa,e 5'-3' orientation as DNA positive strand c. it is made in short fragments d. the template starts at the promoter region e. a, b, and e. a, b, and d
AUG a. is only used as the start codon b. codes for methionine c. determines the reading frame d. is one of the stop codons e. a and b e. a and b
X-rays a. have no effect on DNA b. cause thymine trimers c. cause single and double strand breaks d. make the DNA radioactive c. cause single and double strand breaks
DNA repair mechanisms occur a. only in prokaryotes b. only in eukaryotes c. in both eukaryotes and prokaryotes d. in neither eukaryotes or prokaryotes c. in both eukaryotes and prokaryotes
Of which strand would UV radiation have the most effect? c. AATTAG
Chemical mutagens that minic the naturally occurring bases are called a. nitrogen mustards b. alkylating agents c. base analogs d. nitrous oxide c. base analogs
Gene transfer that requires cell-to-cell contact is a. transformation b. competency c. conjugation d. transduction c. conjugation
The gene for human insulin has been successfully cloned in a. S. aureus b. yeast c. E. coli d. rhinovirus e. virus c. E. coli
Genetic engineering a. allows the use of bacteria as production factories for a # of molecules b. relies on recombinant DNA technology c. is dependent on RNA enzymes d. relies completely on conjugation e. a and b e. a and b
Digestion of DNA by restriction enzymes a. produces sticky ends b. produces blunt ends c. cuts both strands of the DNA molecule d. generates restriction fragments e. all of the above e. all of the above
An ideal vector a. may be a plasmid or bacteriophage b. has a restriction enzyme recognition site c. contains an origin of replication d. contains a selectable marker e. all of the above e. all of the above
Which is true about the RNA transcript? a. formed using the DNA minus strand as a template b. has the same 5'-3' orientation as the DNA positive strand c. made in short fragments d. template starts at the promoter region e. a,b, and d e. a,b, and d
The transcription terminator a. results in a hairpin loop structure in RNA b. results in the polymerase falling off the DNA c. stops DNA polymerase d. adds a terminator nucleotide to the RNA e. a and b e. a and b
The first amino acid in virtually all proteins in bacteria is a. guanine b. methionine c. threonine d. lysine b. methionine
A stop codon a. codes for the stop amino acids b. forms a hairpin loop forcing the ribosome to fall off c. codes for no amino acids d. enhances the binding of the polymerase e. a and b c. codes for no amino acids
Created by: cedes6150