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molecular genetics 1

molecular genetics

QuestionAnswer
Prions not cells, not viruses, misfolded versions of a protein normally found in brain, infectious, cause fatal brain disease, ex- mad cow
Transposons transposabel genetic elements, AKA jumping genes, may jump from from one part of genome to another OR make copies of themselves adn move to another region of teh genome
Barbara McClintock discovered transposons, corn genetics-genes are transposable -- they can move around -- on and between chromosomes, drew this inference by observing changing patterns of coloration in maize kernels over generations of controlled crosses.
Human Genome 3 billion base pairs of DNA mapped
Junk DNA noncoding DNA: regulatory sequences, introns, repetitive sequences
telomere repetitive DNA sequences within a gene
Recombinant DNA DNA from 2 sources combned into 1 molecule
biotechnology or genetic engineering branch of science that uses recombinant DNA
cloning genes may be used to produce protein product-insulin; replace a nonfuctionaing gene- gene therapy; multiple copies of a gene- for analysis; engineer bacteria rep
vector cell that wil carry plasmid/gene
restriction enzymes extracted from bacteria, cut DNA at specific recognition sites
restriction fragments result of cuts made by restriction enzymes, "sticky ends" join
gel electrophoresis separates lage DNA molecules on the basis of movement though agarose in an electric field.
cathode negative end of electrical current
anode positive end of electrical current
negativity of DNA due to presence of phosphate groups
may be altered to allow for finer separatin of small sections of DNA concentration of agarose gel
Polymerase Chain Reaction cell free, piece of DNA can be copied rapidly and used for research
Restriction Fragment Length Polymorphisms(RFLPs) differences in the noncoding regions of DNA between individuals, can be converted into a DNA fingerprint
Created by: adcteacher
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