Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
microlabexam1
| Question | Answer |
|---|---|
| Which test converts various carbohydrates to mixed acids? | carbohydrate fermentation |
| what does a reddish tint indicate in an agar slant before innoculation? | it contains a pH indicator (a pH sensitive dye - phenol red) |
| In testing for pH, what do the resulting colors mean? | yellow means pH below 6.8 ->acidic Red -> alkaline |
| what does a fuschia color in a pH test indicate | pH above 8.2 |
| What agar nut | |
| kliger's iron agar is used for | the differentiation of members of the Enterobacteriaceae on the basis of their ability to ferment dextrose and lactose and to produce sulfides |
| whenever sugars like glucose and lactose are metabolized one of the major endproducts is | ACID |
| when proteins are metabolized (remember proteins are made of amino acids)and when amino acids are broken down | amine groups are released. When amine groups are released, they cause the environment to become basic (alk) |
| If your bacteria are metabolizing protein, how will that be indicated in the test? | The specimen will turn red |
| breaking down of sugar produces | acid |
| waste product of sugar metabolism | acid -> yellow |
| What is NO3? | Nitrate |
| What is produced when NO3 is reduced? | Nitrite (NO2), nitrogen gas, ammonia is produced |
| N2 | Nitrogen gas |
| NH4 | ammonia |
| If the bacteria metabolizes sodium thiosulfide, what happens in the test tube? | It produces a black precipitate |
| What is a product of sodium thiosulfide metabolism that is relevant to our studies? | hydrogen sufide gas -> H2S |
| Gram-negative bacteria are resistant to _____________ which is an important fact to know in Dr. treatment against bacterial infections | penicillin |
| useful for staining the capsule of certain microorganisms. Helps to determine virulence-degree to which a pathogen casues a disease | negative staining |
| staining that requires heat to drive the stain into the wall of an endospore | Endospore staining |
| differentiates bacteria into disinctive groups. | Acid-fast stain |
| a special resistant, dormant structure formed to protect a bacterium from adverse environmental conditions. | Endospore |
| After the mordant is added in a Gram stain, what color are the gram (-) cells? | Both are still purple at this point |
| after the acetone is added in the gram stain procedure, what color are the gram (+) and gram (-) cells? | (+) cells are purple; (-) cells are colorless |
| When viewing cells in a microscope the gram (+) cells will be _____________ and the gram (-) cells will be _________________ color | very purple; pink, pinkish, hot pink, |
| Which test is used to determine the presence of oxidase enzymes. | The oxidase test |
| A_________ is a "substance or compound that is added to a system in order to bring about a chemical reaction, or added to see if a reaction occurs." | A reagent |
| If something is positive for oxidase what will the result be? | black, purplish |
| Most gram (___) bugs have oxidase | positive |
| determines ability of an organism to hydrolyze starch | starch hydrolysis test |
| __________ is an enzyme that catalyses the breakdown of starch into sugars. It is present in human saliva, where it begins the chemical process of digestion | amylase |
| A liquid in a test tube is considered a _______ | broth |
| what are the different types of media? | |
| The first person to observe living microbes in suspension | Antoni van Leeuwenhoek |
| conversion of various carbohydrates to mixed acids | carbohydrate fermentation |
| Uses Kliger's Iron or triple sugar iron agar to test for this | detection of glucose & lactose fermentation, detection of hydrogen sulfide |
| Test for the presence of an enzyme that breaks down hydrogen peroxide into oxygen and water | catalase test. |
| _________ is an enzyme the breaks down hydrogen peroxide into oxygen and H2O | Catalase |
| a test that determines if bug is abl eto use citrate as the sole source of carbon for metabolism | citrate |
| Amylase hydrolizes ___________ to form __________ | starch, glucose |
| NO2 is ______________ | Nitrite |
| refers to the ability of lenses to reveal fine detail or two points distinctly separated | resolution or resolving power |
| Amount the light bends | refractive index |
| ________ refers to rod-shaped bacteria | bacillus |
| __________ refers to becteria with a sperical shape | coccus |
| The three shapes of bacteria are | rod (bacili), cocci, spiral |
| If objects are dark and the field is light, it is _____________ microscopy | Brightfield |
| In ___________ microscopy, small differences in the refactive properties of the objects and the aqueous environment are transformed into corresponding variations of brightness | phase-contrast |
| Not true motility, but rather movement caused by molecules in the liqui striking an object and causing the object to shake or bounce | Brownian movement |
| Suspend infusions by shaking carefully, using loop transfer drop onto slide, place coverslip over drop. this is ______________ technique | wet-mount |
| A depression slide and petroleum jelly are used in the _________________ procedure | hanging-drop procedure |
| an extract from marine red algae; not easily degraded so it remains solid during microbial growth | Agar |
| nutrient broth + agar | nutrient agar |
| most common sterilization tecnique | autoclaving (steam sterilization) |
| microbes which are intentionally introducted to media | inoculated |
| if a broth is cloudy it is considered ________ | turbid |
| A population of cells that arises from a single bacterial cell | colony |
| a "membrane" across the surface of a broth. | Pellicle |
| clumps of microbial cells | flocculent |
| microbial cells which have settled on the bottom of the tube | sediment |
| bacterial growth is determined by ___________________ in a broth? | turbidity, pellicle, flocculent, sediment |
Created by:
microwoman
Popular Biology sets