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Bio 1 quiz 4

QuestionAnswer
cells are the fundamental units of life
Schlerdernt and Schwann creators of cell theory
who created first microscope Robert Hook in England
when was the first microscope created and first cell scene? 1665
large surface volume of cells allows transport easily
GIGO goodies in garbage out
types of microscopy light microscopy and electron microscopy
magnification increase size of object
resolution minimum distance clearer image
smallest resolution in microscope blue light - 0.2 Um (micrometers)
vesicles transport material outside of the cell
lysosome breaking down structures and cells taht the cell doesn't need
plant cells have to ability to perform photosynthesis
phagocytosis ability of cells to secrete products
secretion puts something outside of the cell
unaided eye - how small you can see things you can see , an egg, frog, egg up until .2mm
light microscope - how small you can see 1/2 micrometer. plant and animal cells. nucleus, bacteria, mitochondria
electron microscope- how small you can see 0.1 micrometers. smallest bacteria, viruses, ribosomes, proteins, lipids, small molecules , atoms
cell fractionation break open cell and seperate components to observe
how to fractionate a cell homogenate: blend it ,put in centrifuge get it to seperate into supernatant and pellet
pellet rich in nuclei and cellular debris, depending on how fast it is centrifuged.
prokaryotic cell unicellular
parts of prokaryotic cell flagellum, cell wall, capsule, dna, ribosomes, plasma membrane
con of having one big cell lower surface area ratio
surface area height x width x number of sides x number of boxes
electron scope vs light microscope electron microscope is 1000x better
resolution of unaided eye 0.2mm
resolution of light microscope 200 nm
resolution of electron microscope 0.2nm
cell fractionation step 1 homogenization - blender
cell fractionation step 2 seperate cell based on cell
cell fractionation step 3 centrifuge - faster spin more g force.
where did big cells go when you centrifuge to the bottom, pellet, they don't need high velocity to be seperated
plasma membrane - prokaryotic cell surrounds cytoplasm
DNA- prokaryotic cell contains genetic information for reproduction. no nucleus
ribosomes protein synthesis of enzymes ( metabolism)
cytoplasm inside cell
cell wall outside of cell
Created by: michelle5671
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