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Developmental Bio

Test 1

QuestionAnswer
Evolutionary Biology Seeks the understanding of how organisms evolve over time
How many cells in human body? 210 different types of cells (10-75 trillion cells in body)
Gametogenesis production of gametes in germ cells via meiosis (1N gametes) It is an orderly program of changes in cell shape and form. Activation of certain sets of genes and suppression of others (in animals, gametes are produced in gonads)
non-equivalent cell division the reason why a single cell can give rise to a creature composed of several different specialized cell types, yet all having same genes.
Progeny two cells that have the same genes, but are not exactly the same
Cell Communication needed to coordinate development process
Diploid chromosome number is 2N (46 chromosomes)
Haploid chromosome number is 1N (23 chromosomes)
Meiosis involves 2 divisions and reduces chromosome number (produces gametes) Starting with a single germ cell, 4 gametes are produced
Diplontic Life Cycle Life cycle where the dominant life cycle is diploid (humans). The only haploid life cycle within these organisms is within the gametes
Diplohaplontic Life Cycle alternating generations between both multicellular phases of haploid and diploid (mosses and ferns)
Haplontic Life Cycle Haploid phase dominates life cycle, mitosis actually results in the production of gametes. The single-celled zygote production is the only time these organisms go through the diploid phase.
Cell Division is responsible for: 1) Development and Specialization: lots of differentiation must occur, non-equivalent cell division occurs here 2) Growth: don't want differentiation 3) Maintenance: replace old cells with new ones
Mitosis division of nuclear material
Cytokinesis: division of the cytoplasm, often referred to as cleavage in animals. Begins with formation of cleavage furrow
Cell cycle period of time from 1 division until the next. Divided into two general phases (M Phase and Interphase)
Interphase further subdivided into 3 parts: S Phase (DNA synthesis/replication), G1 Phase, and G2 Phase
Ligands Organic Molecule involved in signaling
Cell Signaling Connections In plants: plasmodesmata in animals: tight junctions, desmosomes (not direct connections), gap junctions (direct channels from cell-to-cell)
Endocrine hormonal signaling
Paracrine one cell to many cells
Neuronal neuron signaling in nervous system
Contact-Dependent one cell to one cell
3 Stages of cell signaling Reception, transduction, response
Reception certain proteins in the target cell receive their signals (one protein, one signal) Target cell detects ligand when ligand binds to receptor protein
Transduction receptor protein change with binding of ligand, initiates transduction, often a sequence of steps
Response transduced signal triggers a specific cellular response
Dogma of modern biology each cell generated by mitosis has same chromosomes (two homologs of each kind) and each chromosome has same genes and regulatory regions
Gene Regulation why some cells develop into different tissues
Asymmetric Division sister cells are born different
Symmetric Division sister cells become different as result of influences acting on them after their birth
Cell Lineage the developmental pathway and fate of cells
Position where one cell is relative to another
Early embryogenesis including cleavage
later embryogenesis cell proliferation within developmental tissues
Patterning Establish positional identities within embryo
Morphogenesis change in form, gastrulaton, formation of 3 germ layers (mesoderm, endoderm, ectoderm) that define tissue layers
Cell Differentiation cells become structurally and functionally distinct. Takes place later in development.
Proteins Genes are expressed through genes
Cell Activities: expressed through proteins
Developmental Processes regulated by cell activities
Transcription RNA
Post-transcription mRNA
Translation Polypeptide
Post-translation Active molecule
Apoptosis program cell death, certain periods of development have cell death, not all completely growth
Blastula A hollow ball of cells that turns into a gastrula
Gastrula several layers (germ layers) of cells filling in the blastula
Oogenesis Development of the egg, growth and development of oogonia to produce mature egg
Spermatogenesis maturing of sperm
Oogonia germ cell within the ovary
stem cells undifferentiated cells capable of giving rise to highly differentiated progeny and reproducing via meiosis.
Oocyte the oogonium (oogonia) grows into this
1st Meiotic Division results in secondary oocyte + 1 polar body
2nd Meiotic Division results in Ootid + 1 polar body
Follicle germ cell + 1 or more layers of "nurse" or follicle cells
Nurse cells give the germ cells nutrients to support life
Primary Oocytes primary follicles
Secondary follicles develop as oocytes continue to grow
Antrum spaces between follicle cells
Graafian Follicle from this the egg ovulates, contains lots of liquor called folliculi
Corpus luteum the endocrine organ that secretes horomes (progesterone and estrogen) If pregnancy does not occur, the corpus luteum is replaced by scar tissue
Testis male gonads
sperm primordial germ cell
Seminiferous tubules where the sperm is held
spermatogonia the process in which the germ cells undergo mitosis
Primary Spermatocytes the germ cells that enter meiotic pathway
Spermatogenic cells (spermatocytes) the cells between the seminiferous tubules
Spermatogonia germs cells going through mitosis
1st Mitotic Division of Sperm results in: 2 equal sized secondary spermatocytes
Spermatid the secondary spermatocyte
Spermatozoon internal reorganization of spermatid, develops into mature sperm
spermiogenesis the transition process from spermatid to spermatozoon
3 Parts of sperm Head, midpiece, tail
Zygote sperm + egg
Syngamy fusion of two haploid nuclei from sperm and egg
acrosomal filament specific receptor on egg surface (proteins in tip of sperm are recognition proteins)
Fate Map Eventual path of cells
Blastulation blastula structure, and subsequent development, we start to see major differences that begins to define the uniqueness of major groups.
Gastrulation development of germ layers in early embryo, initial stages of morphogenesis and organogenesis, significant cell and tissue movement begins. Patterns begin to become distinct.
Cleavage cells blastomeres
Morula cleavage embryo (early embryo made of many blastomeres)
synchronized cells all develop in order
asynchronized cells are at many different stages
Vegetal Side Yolk-rich side
Animal Side Opposite side of yolk-rich side (usually houses the nucleus)
Microlecithal Eggs Little yolk within egg, often isolecithal
Isolecithal uniformly distributed yolk (all microlecithal eggs are isolecithal, not all isolecithal are microlecithal)
Mesolecithal eggs moderate yolk, moderate size (usually telolecithal, but can still be isolecithal)
Telolecithal asymmetrical distribution of yolk
Megalecithal eggs lots of yolk, large eggs, usually telolecithal, can be centrolecithal
Centrolecithal yolk in center, moslty megalecithal eggs
Holoblastic Cleavage complete cleavage
Meroblastic Cleavage incomplete cleavage, usually due to amount of yolk and size of egg
Radial Cleavage Blastomeres radially symmetrical. Early cleavage spindles in embryo vertical (perfectly symmetrical, orderly)
Spiral Cleavage plane of cleavage shifts, arrangement of mitotic spindles determines plane of cleavage. Mitotic spindles are arranged obliquely rather than vertically. Each blastomere of upper tier lies over junction of two bottom blastomeres.
Dextral Clockwise
Sinistral counter clockwise
Bilateral cleavage bilateral symmetry, some cleave to give rise to bilaterally symmetrical blastula, distinct left and right.
Rotational Cleavage happens in mammals, mitotic axes during 2nd cleavage perpendicular to one another rather than parallel.
Superficial Cleavage cell is multinucleated but not multicellular. Cleaved nuclei migrate to peripheral, activate cytoplasm of egg. Results in syncytium
Discoidal Cleavage little section called blastodisc is where the entire development of the embryo takes place. (little dude with hair)
Polar Lobe Formation protrusion close to vegetal pole, prior to 1st division. Associated with only one daughter cell, segregation of part of cytoplasm into 1 blastomere. Differential distribution of cytoplasm
What is accomplished during cleavage? Build a multicellular embryo
When does cleavage end? Blastulation (start of RNA synthesis, finally beginning to use own genome instead of moms)
Blastocoele the cavity within a blastula
Deuterostomes Blastula is a hollow ball of cells
Protostomes blastula is solid
3 Primary germ layers Ectoderm, Mesoderm, Endoderm
Ectoderm skeletal system and part of nervous system
Mesoderm organ systems
Endoderm Digestive tract and some digestive organs
Archenteron the "punch hole" in the gastrula which becomes the primitive gut of the organism
Invagination sheet of cells (epithelial cells) bends inward
Ingression individual cells leave an epithelial sheet and become freely migrating
Mesenchyme cells Undifferentiated, mobile cells derived from germ layers that leave epithelial sheet during ingression
Involution epithelial sheet rolls inward to form an underlying layer (folds and bends to form multiple layers)
Epiboly sheet of cells spreads by thinning (only finite amount of cells, must be spread out to cover everything)
Intercalation row of cells move between one another, creating array of cells that are longer but thinner
Convergent extension row of cells intercalate, but intercalation highly directional
Phylotypic Stage Stage at which members of animal group, not necessarily whole phylum, show maximum similarity to each other
Vertebrates all have... notocord, neural tube, paired somites, tailbud, brachial arches
Fruit Flies only protostome we will study (drosophilia melanogaster) genetics are very well understood micromanipulations difficult short life cycle, rapid development
Eumetazoans true tissues
Bilateria bilateral symmetry
coelomates body cavity derived from mesoderm tissue
Ring canals cytoplamsic bridges connecting products of early mitotic divisions (divisions from the germ cell)
Germ line cells Oocyte + nurse cell
Somatic cells follicle cells form gonads
Syncytium embryo with all nuclei in common cytoplasm
Segmentation adult body plan arranged as segments: Head, thorax, and abdomen
Parasegments segmentation in thorax and abdomen that do not go through the entire body
Morphogens a molecule, usually a protein, found in gradients within cells or organisms, indirectly regulate other developmental processes
Bicoid Anterior, defines head
Nanos Posterior, defines anus
Gap Genes embryonic genes, affect later stages in development, regulated by maternal effect genes
Pair-rule genes initiates early pattern, early segmentation
Segment polarity genes clearly defining segments, top and bottom is shown
Homeotic (selector) genes found in homeo boxes, a common sequence on chunks of DNA, regulate different aspects of development
Regulatory 'Cascade' essentially "building" n embryo
Created by: stevanie
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