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BioLab Vol 1 P2
Practical 2
| Question | Answer |
|---|---|
| Enzymes are? | Organic catalysts that speed metabolic reactions. They are proteins. Therefore they can be affected by pH and temp. They are specific and speed only on type of reaction. |
| What does Catalase do? | Catalase is the specific enzyme for hydrogen peroxide. it is used by cells to rapidly catalyze the decomposition of hydrogen peroxide to less reactive oxygen and water. |
| What is the effect of Temperature on enzyme Activity? | Cold temperatures slow chemical reactions. Warm temperatures speed chemical reactions. Boiling temperatures denature enzyme and change it's shape |
| Does more enzyme or more substrate speed up a reaction? | The higher enzyme or substrate concentration results in faster enzyme activity. |
| What happens when you change the pH of a solution? | Acidic pH-enzyme shape is changed Neutral pH-optimum pH for reactions Basic pH-enzyme shape is changed |
| What is required for for aerobic and anaerobic respiration? | Glucose is generally required. |
| What is produced by aerobic respiration? | Fermentation is taking place and is producing ethanol + Carbon dioxide + 2ATP in yeast and lactate + 2ATP in animals |
| What is produced by anaerobic respiration? | cellular respiration is taking place and is producing Carbon dioxide + Water + 36or38 ATP |
| What happens when you mix: Yeast + Glucose Yeast + Fructose Yeast + Sucrose Yeast + Water | Glucose-Anaerobic-monoasaccaride it is easily broken down. Fructose-Anaerobic-monosaccaride-Easily transformed into Glucose Sucrose-Anaerobic-disacciride-used less effectively, process is slow Water-nothing |
| What is produced by fermentation? | Either Ethanol+Carbon Dioxide or Lactate is produced. |
| What is produced by Aerobic Respiration? (Cellular Respiration) | Carbon Dioxide + Water |
| Aerobic Cellular Respiration Formula | Glucose + (6)Oxygen Gas -----> (6)Carbon Dioxide + (6) Water + 36 or 38 ATP |
| Photosynthesis Formula | (6)Carbon Dioxide + (12)Water -----> Glucose + (6)Water + (6)Oxygen Gas |
| What is required for photosynthesis? | Light |
| What is produced by photosynthesis? | Plants take in Carbon Dioxide and release Oxygen Gas and Water. Producing Carbohydrate and Oxygen. |
| Where does photosynthesis occur? | In the chloroplast. |
| Where do light reactions take place? | In the Thylakiods in the chloroplast. |
| Where do light-independent reactions take place? | In the Stroma in the chloroplast. |
| Can light reactions or light-independent reaction happen in the dark? | Light reactions and Calvin Cycle stop in the dark because ATP synthesis and NADP(pos) reduction require light. |
| What is a pigment? | Pigments are molecules that absorb wave lengths in visible range. |
| Name the pigments in order from top-most to lowest. | Carotene -Yellow Xanthophyll -Yellow orange Chlorophyll a-Blue green Chlorophyll b-Yellow green |
| What happens in plants in the light? | Photosynthesis |
| What happens in plants in the dark? | Cellular Respiration |
| What happens to green plants in green light? | Cellular respiration because the plant can not absorb the green light so it believes that it is dark. |