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Micro Lab Ex 1

Exam 1

QuestionAnswer
BSL-1 org not cause disease minimal threat to environ & personnel standard microbio practices adequate can handle in open, no special containment
BSL-2 orgs commonly encountered in community moderate environ &/or health hazard assoc w/ human disease of varying severity can do splashless or non-aerosol using standard microbio procedures
BSL-3 orgs of local or exotic origin assoc w/respiratory transmission & serious or lethal disease special ventilation systems, restricted access special training, use Class I or II biological safety cabinet,not open bench
BSL-4 orgs great potential for lethal infection inhalation, droplet exp, autoinoculation lab isolated, access strictly controlled ventilation & waste mngmnt controlled special training,Class III or II (w/ +press body suit & life support)cabinet
Where are sharps containers? large glass-by the hood and rear exit, small-front lab bench, rear exit
Where is the first aid kit? By the biohazard container
Where are the shower and eye wash? near exit at the back
Where are fire extinguishers? by exit at front lab bench, by back exit
What is a controlled experiment? All variables are constant except experimental variable,only way to make sure results are reliable
In microbial terms, what does sterility mean? Free of contaminants (i.e. all living orgs)
Agar is an extract of what plant? seaweed
What is an autoclave used for? sterilization of equipment and media that cannot be damaged by heat
Agar solidifies at what temp? 45 degrees C
Name 3 natural sources we can sample for microorgs. air in public bldg, soil, water
What is the objective of a streak plate? to spread bacteria on surface to isolate it
On a streak plate or in a pour plate, a colony develops from how many microbial cells? 1
What are you trying to achieve when performing a serial dilution technique (what is purpose of serial dilution?)? reduce concentration of microbes
What must be done to get agar to melt when mixed in water, or at approximately what temp does agar melt? 100 degrees C; boiling water
Which plate has fewer colonies? 1/10, 1/100, 1/1000 dilution 1/1000
What do you do with materials you used in lab and to work surface? plates to biohazard bin, broken glass to box, full glass tubes to hood, sharps in biohaz on counter, instructor special instructions; wipe w/ water, disinfect w/ lysol
What is the condenser lense? lens mounted in or below stage, focus or condense the light onto the specimen, increases illumination and resolution
What does a cover slip do? thin square piece of glass or plastic placed over specimen on a slide, when used with liquid samples it flattens liquid and assists with single plan focusing
What is depth of field or depth of focus? depth or thickness of the image space that is simultaneously in acceptable focus.
What is a diaphram? used to adjust the amount of light reaching specimen
What is field of view? FOV, diameter of the circle of light that you see when looking in a scope, as power gets greater, FOV gets smaller
What is parcentered? alignment issue, whn changing from one objective lens to another the image of the object should stay centered
What is parfocal? specimen remains in or near focus when you change objectives
What is resolution? ability of a lens system to show fine details of the specimen
What is working distance? dist betw objective and specimen when image in focus
What is inversion? image is seen as reversed and upside down when passing through a magnifying lens
What is refraction? ability to bend light
What is magnification? increasing the size of an image
What does the objective do? magnifies specimenand creates real image
What does the ocular do? view specimen and magnify, usually 10x
What does the revolving nosepiece do? can change objectives without damaging them
Created by: monchichi521
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