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Bio Practical

First two labs

QuestionAnswer
What is a Spectrophotometry Analysis of substances based on their ability to absorb specific wavelengths of light. It permits photometric readouts in transmittance, absorbance and concentration. Standard unit is nm=1X10-9m
Five steps of Spectrophotometry 1. Let it warm up for 5-10 min, plug in, turn on select wavelength 2. Label Cuvettes, glass test tubes on top 3. Make a blank 4.Each time change wavelength blank it 5. Wipe down with kim wipes
What is a Blank used for To cancel out any other effects that the other components in the solution may have on the absorbance readings
Wavelength where substance has highest absorption lambda max (h max)
How do you read a glassware's volume Meniscus at eye level
What are Transfer pipets? 1. Transfer Pipet (sim to Pasteur Pipet) Made of plastic has a built in bulb, for dropwise, not accurate. Use for one solution then dispose
What are Graduated Pipets? 2. Graduated Pipet- Specific volumes, divisions imprinted in discrete units, (10th of milliliter or hundreth ml .01, 0 @ barrel, final volume at tip. Must use right size -> Requires Pipet Pump to draw liquid into pipet.
What are Volumetric Pipets? Volumetric Pipets: fixed/variable volumes (0-20 ul, 20-200 ul, 200-1000ul) Range is indicated on it, amount dispensing shown in window. Do not adjust volume or go beyond max. Use disposable plastic tips; remove each after use. To change vol turn dial.
How do you use the Volumetric Pipet? To pick up a liquid: Push to 1st stop in AIR. In water, push fully To drop off liquid: Push to 2nd stop, then remove tip
Created by: 523971751
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