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Chapter 3 vocabulary
Microbiology
| Question | Answer |
|---|---|
| Dark-field microscopy | light coming directly through specimen is blocked out, so that the light reflected off the bacteria is seen. Makes structures appear against a black background and seem bigger than they really are. |
| Differential stain | differentiates between 2 common types of bacteria |
| Electron microscopy | viruses are stained with a heavy metal and electrons are shined on them--electrons are more powerful than photons--can resolve down to 0.002 microns, to show even the tiniest of virus particles. |
| Medium | a nutritious extract or mixture of materials that will support the growth of microbes. |
| scanning electron microscopy | scattered electrons are detected and the object's surface is reconstructed by computer technology.--good for visualizing surface structures--resolves to about 0.02 microns |
| Staining | dye added to the organisms to improve contrast and our ability to recognize the microbes--stained bacteria have a more apparent shape. |
| Transmission electron microscopy | the electrons pass through the specimen. Heavy metals pile up around the virus and scatter electrons. This leaves dark areas that reflect the viral outline on the veiwing screen. |