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Microbiology set 1
Exam 1
| Question | Answer |
|---|---|
| Leeuwenhoek? | Made the simple microscope? |
| Robert Hooke? | Created the compound microscope |
| J. M. Berkeley | (1845): Potato Blight of Ireland caused by a fungus (Phytophthora infestans) |
| Pasteur | silkworm disease etiology caused by a protozoan |
| Helicobacter pylori & Oral streptococci | gastric / stomach ulcers; stomach cancer Heart valve diseases |
| Semmelweiss | Childbirth fever deaths hand washing in OB ward |
| Joseph Lister | antiseptic techniques for surgery; carbolic acid (phenol) is still used as an index of effectiveness of antiseptics (phenol coefficient) |
| Ivanovski | Tobacco mosaic virus |
| Robert Koch | Made nutrient rich media from extracts from natrual materials such as meat and plant materials. koch posstulates. |
| 3 Main Domains in Microbacteria? | bacteria, archaea, eukarya |
| types of bacteria | blue/green algae and cyanobacteria |
| types of archaea | Halophilic Methanogenic Acetogenic |
| what does Halophilic, Methanogenic, Acetogenic mean? | Halophilic (can tolerate salty environments) Methanogenic (produce methane) Acetogenic (acetic acid producers) |
| types of eukarya | fungi, algae, protozoa |
| how are fungi characterized | Sexual reproductive mode Morphology Biochemistry Ecology |
| how are algae characterized | pigment colors |
| how are protozoa characterized | mode of motility |
| examples of sub-viral entities? | viroids and prions |
| sizes of viruses, bacteria, protozoa | Viruses- 0.03-.3 um Bacteria- .1-10um Protozoa and fungi- 4-40um |
| swan neck flask | bent glass rod leading into a test tube and significantly slows down the action of bacteria spreading through into the test tube. |
| sawn neck flask | help prove that growth is not spontaneous |
| taxonomy name for tuberculosis | Mycobacterium tuberculosis |
| taxonomy name for anthrax | Bacillus anthracis |
| koch postulate #1 | Microbe present in every case of the disease but absent in healthy hosts |
| koch postulate #2 | Suspected microbe can be isolated and grown in pure culture |
| koch postulate #3 | Same disease must be produced when a healthy host is inoculated with that microbe |
| koch postulate #4 | Same microbes must be isolatable from the newly diseased host |
| Imitations of Koch’s postulates? | Viable but non culturable microbes and unethical testing |
| genetic material in pro and eukar | pro: usually found in single circular chromosome eukar: found in paired chromosome |
| location of genetic material n pro and eukar | pro: nuclear region (nucleoid) eukar: membrane-enclosed nucleus |
| nucleolus n pro and eukar | pro: absent eukar: present |
| histones n pro and eukar | pro: absent eu:present |
| mitotic spindle n pro and eukar | pro: absent eu: present |
| plasma membrane n pro and eukar | pro: lacks sterols eu: contains sterols |
| internal membranes n pro and eukar | pro: only in photosynthetic organism eu: numerous membrane-enclosed organelles |
| endoplasmic reticulum n pro and eukar | pro: absent eu: present |
| respiration (ATP) n pro and eukar | pro: at cell membrane eu: in mitochondria |
| golgi n pro and eukar | pro: absent eu: present |
| lysosomes n pro and eukar | pro: absent eu: present |
| peroxisomes n pro and eukar | pro: absent eu: present |
| ribosomes n pro and eukar | pro: 70s eu: 80s in cytoplasm and on endoplasmic reticulum, 70s in mitochondria |
| cytoskeleton n pro and eukar | pro: absent eu: present |
| cell wall n pro and eukar | pro: peptidoglycan, LPS and teichoic acid eu: none in most eukaryotic cells, chitin in fungal cells |
| external layer n pro and eukar | pro: capsule or slime layer eu: none in most eukaryotic cells, pellicle or shell in certain parasites. |
| cilia n pro and eukar | pro: absent eu: present in certain cell types |
| pili n pro and eukar | pro: present eu: absent |
| flagella n pro and eukar | pro: present eu: present in certain cell types |
| cell division n pro and eukar | pro: binary fission eu: mitosis or meiosis |
| reproduction mode n pro and eukar | pro: asexual eu: sexual or asexual |