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BioLab Vol 1 Lab 2

Biology Lab Manual Vol 1

QuestionAnswer
Stereomicroscope (Dissecting Microscope) allows you to view objects in three dimensions at low magnifications. it is used to study entire small organisms. produces a three dimensional image.
Compound light microscope used for examining small or thinly sliced sections of objects under higher magnification. uses two sets of lenses and light to view an object.
compound refers to the use of two sets of lenses. the ocular lenses located near the eyes and the objective lenses located near the object.
transmission electron microscope is analogous to the compound light microscope. The object is ultra-thinly sliced and treated with heavy metal salts to improve contrast.
scanning electron microscope is analogous to the dissecting light microscope. it gives and image of the surface and dimensions of an object.
resolution is the minimum distance between two objects at which they can still be seen as two separate objects.
Binocular head Holds two eyepiece lenses that move to accommodate for the various distances between different individual's eyes
Eyepiece lenses The two lenses located on the binocular head.
Independent focusing eyepiece allows independent adjustment of each eye
Fixed eyepiece The non-adjustable eyepiece
focusing knob A large, black or gray knob located on the arm; used for changing the focus of both eyepieces together.
Magnification changing knob A knob, often built into the binocular head, used to change magnification in both eyepieces simultaneously. This may be a zoom mechanism or a rotating lens mechanism of different powers that clicks into place.
Illuminator Used to illuminate an object from above; may be built into the microscope or separate.
Viewing head Holds the ocular lenses.
Arm Supports upper parts and provides carrying handle.
Nosepiece Revolving device that holds objectives.
Objectives Scanning objective Low-power objective High-power objective Oil immersion objective
Scanning objective This is the shortest of the objective lenses and is used to scan the whole slide.
Low-power objective This lens is longer than the scanning objective lens and is used to view objects in greater detail.
High-power objective If your microscope has three objective lenses, this lens will be the longest. It is used to view an object in even greater detail.
Oil immersion objective (on microscopes with four objective lenses) Holds a 95x (to 100x) lens and is used in conjunction with immersion oil to view objects with the greatest magnification.
Stage Holds and supports microscope slides. a mechanical stage is movable stage that aids in the accurate positioning of the slide.
Course-adjustment knob knob used to bring object into approx focus; used only with low-power objective.
Fine-adjustment knob Knob used to bring object into final focus
condenser Lens system below the stage used to focus the beam of light on the object being viewed.
Diaphragm (Diaphragm control lever) Controls amount of illumination used to view the object.
Light source An attached lamp that directs a beam of light up through the object.
Base the flat surface of the microscope that rests on the table.
Field of view Through a microscope - the circle visible through the lenses.
Diameter of field the length of the field from one edge to the other.
Wet mount prepared by placing a drop of liquid on a slide. objects should always be viewed this way.
longitudinal section a lengthwise cut through any type of specimen.
cross section A crosswise cut through any type of specimen.
Meniscus The curve in the upper surface of a liquid close to the surface of the container or another object, caused by surface tension.
Created by: banken
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