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Tour of Cell

Chapter 7

QuestionAnswer
What is resolving power for? Image clarity
What is only in a pro cell? Nucleoid- No membrane seperates it from the rest of the cell
Where is DNA located? Nucleoid
What don't pro cells have that euk do? Don't have: Mitochondria, vacuoles, ER, chloroplast, nucleus, cytoskeleton
Are structures in a prokaryotic cell membrane bound? No
What is only in a eukaryotic cell (not in pro)? Chloroplast, Mitochondria, Vacuole, Nucleus, ER, Nuclear Envelope, Cytoskeleton
Where is DNA located in a euk cell? Nucleus
Where are chromosomes located in a euk cell? Nuclear envelope
Are euk cells membrane bound? Yes
Electron Microscopes focus what? They focus through specimen
TEM study what? TEM study internal ultrastructure of the cell
SEM Surface studying - excites electrons on the surface film of gold
What is in both euk and pro cells? Ribosomes, DNA, chromosomes, cytosol, cell membrane
What does plasma membrane contain? Glycoprotein, phospholipid, cholesterol, transport protein
What does a plasma membrane NOT contain? Nucleic acid
What does pro mean? Before the nucleus
An ex of pro cell: Bacterium
If penicillin inhibits bacterial cell wall construction: why does it die? Water enters the bacterial cell- swell and rupture
Cytoplasm of euk cell contains: Water, dissolved nutrients, organelles, enzymes
Passage of materials into and out of the nucleus Is unrestricted for very small molecules, but tight for large
Not DNA Nucleosome
DNA has Chromatin, chromosome, nucleotide, and colored bodies
What is not in nucleus? Mitochondria
If you found a cell with large amounts of ER it could mean: Large quantities of enzymes for biological processes are manufacture by that cell. Nuclear membrane is made by cell. Proteins for export are made.
If you found a cell with large amounts of ER it could NOT mean: Membrane lipids are produced
An organelle capable of synthesis of old or worn out nuclear membrane is the: ER
Not component of euk nucleus: Centrioles
ER makes Lipids and testosterone
What is composed primarily of protein? Cytoskeleton
Which kingdom has organisms with chloroplasts Protista and Plantae
Evidence that Mito could have been free living Produce ATP and contain own DNA
Chlorophyll is found in Thylakoid membrane
In metabolically active cells, expect to find large amounts of: Mitochondria
What does a cell do with a defective organism? Lysosome digests it
Anaerobic metabolism: Occurs in the cytosol
Which part of cell is used for cell division? Cytoskeleton
Actin and myosin are examples of Microfilaments
Actin and myosin are associated with skeletal contractions. These microfils also have been located in All other euk cells
What is in cytoskeleton? Tubulin, Actin, Myosin
What is not associated with cyto? Keratin
The filaments for moving organelles Microtubules
What happens if cilia is removed surface of cell and immediately place in a solution containing energy yielding molecules? The cilia continue to show movement.
Paramecium cannot move- what is NOT a logical hypothesis as to why? It has very few golgi bodies.
Paramecium cannot move- what IS a logical hypothesis as to why? It failed to produce cilia, lacking basal bodies, low # of mito, its microtubules organizing center are not functioning.
Where in a unicellular organism would you expect to find the most mito? Area surrounding basal body.
Cell fractionation separates what? Major organelles of the cell so individual function can be studied
Blender: Homogenizes and breaks up cell and then go to centrifuge to separate into supernatant and pellet
Cells are separated by Centrifuge
Top of solution after centrifugation: Supernatant (lighter molecules)
Bottom of solution after centriguation: Pellet (heavier molecules)
What is the nuclear side of the envelope lined by? Nuclear lamina
What is the nuclear lamina? Network of intermediate fibers- maintain shape of neuc
Within nucleus, the DNA and associated proteins are organized into fibrous material Chromatin
23 chromosomes Sex cells
46 chromosomes A typical human
Nucleolus makes what? Ribosomes
How does nucleolus make ribos? Ribosomal rRNA made and assemble with protein from cyto to from ribsomes
Ribsomes contain what? rRNA and protein
Non dividing cell Chromatin
Dividing cell (spindles) Chromosome
Some ribosomes are suspended in cyto and make proteins that function with cytosol called: Free ribosomes
Other ribosomes attach to ER and the protein goes to golgi apparatus called: Bound ribosomes
Created by: lizthomson
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