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micro lab exam2
pearson microbiology Bauman lab 2
| Question | Answer |
|---|---|
| Blood agar - test type - chemical reaction - and plate changes | differential - hemolysis - breakdown of hemoglobin - greening or clearing of medium |
| Blood agar results Alpha Hemolysis - bacteria example | Incomplete breakdown of hemoglobin - greening of medium - streptococcus bovis |
| Blood agar - results Beta hemolysis - bacteria example | Complete breakdown of hemoglobin - clear zone - Streptococcus pyogenes and staphylococcus aureus |
| Blood agar - results gamma hemolysis - bacteria example | No growth or no breakdown of hemolysis - enterococcus faecalis |
| MSA - full name - uses - test type - initial color | Mannitol Salt Agar - isolation of staphylococcus - differential for mannitol fermentation selective because of high salt - reddish plate |
| PH indicator in MSA | phenol red - yellow acidic red alkaline |
| Results for MSA | Yellow = + mannitol fermentation Orange red = no reaction Hot pink = - for mannitol fermentation |
| DNA agar - test type - initial color - indicator - results - example | Differential enzyme test - light bluegreen - methyl green - if bacteria produces enzyme DNase clearing of medium - halo =+ DNase - Staphylococcus aureus |
| BEA - full name - test type - initial color - indicator - initial color | Bile Esculin Agar - differential esculin breakdown and selective bile and sodium azide inhibits - yellow plate |
| BEA results - example | Black media = (+) esculin breakdown no black = - esculin breakdown |
| Plasma coagulase - test type - contains - results - example | differential enzyme test - rabbit plasma - organism produces coagulase will cause fibrinogen to form fibrin clot - clot = (+) for coagulase - staphylococcus aureus |
| 6.5% NaCl broth - test type - uses | Selective salt tolerance - growth causes turbidity - used to differentiate E. faecalis and streptococcus species - turbidity = (+) clear = (-) |
| Catalase test - test type - results - example | Differential enzyme test - if organism produces catalase it will bubble when exposed to hydrogen peroxide - |
| Methyl red is used to test for _____ and turns what color?? | pH indicator - red is ACID and yellow is alkaline |
| Which three tests show sulfur reduction? | HEA TSI SIM |
| The clear zone around an antibiotic disc is called ______ and if it is under 10mm then the bacteria is ________ to the antibiotic. | Zone of inhibition and resistant |
| EMB - full name - uses - test type - indicator | Eosin Methylene Blue Agar - isolate gram neg - selective eosin m blue and dyes are inhib to gram +; differential lactose fermentation - colors of colonies |
| EMB results - example | Metallic green/blue black colonies (+) vigorous lactose fermentors, acidic Dark purple colonies = (+) slow fermentors lt pink colorless colonies = (-) fermentation -e coli blue black metallic green |
| Phenol red broths test for and contain ________ tubes | carbohydrate fermentation with or without gas - Durham tubes |
| Phenol red broth indicator - results | Phenol red - yellow is acidic pink is alkaline A - acid (yellow) = (+) carb fermentation NR = no reaction K - alkaline (pink) = (-) carb fermentation G - gas in durham tube |
| SIM - full name - test type - tests for | Sulfide Indole Motility - Differential - production of hydrogen sulfide, motility and breakdown of tryptophan |
| What is added to a SIM test to test for indole? What color does it change to? What does it mean? | Kovac's reagent turns red if the organism is able to breakdown tryptophan and produce indole |
| SIM results - example of result | Black = (+) H2S no black = (-)H2S Red kovac's = (+) indole yellow Kovac's = (-) indole Motility = cloudy funnel shape S+ I+ M- |
| What method of inoculation do you use for a SIM test? | Stab |
| Urea broth - test type - looks for - indicator - results | Differential enzyme test - urease will break down urea into ammonia and Co2 which makes it alkaline - indicator is Phenol red - Hot Pink = (+) rapid urease Peach = (-) urease |
| Phenylalanine slant - test type - special content - initial color - looking for - results | Differential enzyme test - amino acid phenylaklanine and a reagent FeCl3 - blue - PDase will deaminate phenylalanine and produce ammonia Dark Green = (+)for PDase No change = (-) for PDase |
| Citrate slants - test type - initial color - indicator - results | Differential enzyme test - blue - bromthymol blue - Blue = (+) for citrase Green = (-) for citrase |
| What is the nitrogen source in a citrate slant? | ammonium phosphate |
| What is the carbon source in a citrate slant? | sodium citrate |
| What does a color change from green to blue in a citrate test indicate? | An organism producing the enzyme citrase will convert the nitrogen and carbon sources into pyruvic acid and ammonia which will cause a pH shift to alkaline and change from green to blue. |
Created by:
hkrawietz
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