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Microbio Lab 4

CU microbiology Lab Quiz 4

QuestionAnswer
when determining plate counts, what are the number guidelines? 30-300
From step one of lab 27, know how to determine dilution factors. when starting from a solution it is always even and starting from a solid mixture is odd numbers....
From step four of lab 27, how do you accomplish this? it equals the positive opposite.
28, know how o measure the absorbance of a broth culture. Shake it up and the absorbance measures the amount of light transmitted through a turbid broth culture.
28, know the results step 2 which is dealing with the graph. When E. Coli(underlined) is read it is in __X10^8
29, Know the medium used and the organism tested for. medium = Synder test agar Organism= Streptococcus mutans (Underlined)
30, WHy was each incubation period used? to determin between psychrophile, mesophile, and thermophile
30, Make sure you can do step 3... measure standard plate count
31, Be able to measure the MPN of a sample, The most probable numbers. (look at the bubbles) look at a sheet and by reading the 15 different samples defined by 10mL 1mL and 0.1mL, and see how many are positive with Bubbles
31, procedure of day 3: observe the plates to see if you can identify E.coli The E. coli will be small with a green metallic sheen.
31, introduction: The presumptive test, is a positive production of gas iin lactose broth within 24 hours
31, introduction: the confirmed test will show typical coliform colonies on Eosin-Methylene Blue agar (EMB)plates. E. coli colonies usually are dark with a green metallic sheen. E. aerogenes colonies are larger and lighter in color.
31, what is the standard procedure for testing for coliformsand why is it used? the presumptive test, the confirmed test, and the completed test
32, be able to interpret the results of an enterotube incubation. Kovac's reagent, a drop, goes in the indole section. locate 5 digit number in the book.
Created by: jseekins
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