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UOP Microbiology

Lecture 24: Food Microbiology

QuestionAnswer
extrinsic factors factors involved with the processing/ handling of food; not the food itself temperature, humidity, pressure
perishable foods tend to be nutrient rich, moist, and unprotected by covering (rind) need to be refrigerated; Ex) milk
semi-perishable foods can store in sealed container for months without spoiling; many fermented foods
non-perishable foods usually dry or canned foods that can be stored almost indefinitely without spoiling
high protein food spoilage proteolysis/putrefacation, release smelly amines like cadaverines
high fat food spoilage lipid hydrolysis rancid fatty acids
high carbohydrate spoilage first signs indicated by fungi, not smelly
Erwinia carotovora cause fruits and vegetables to soft rot because it produces hydrolytic enzymes
low pH favors molds or yeasts Ex) orange
high pH favors bacteria Ex) meat = alkaline
water activity arbitrary number assigned on how moist something is
osmophillic organisms can tolerate high salt/ sugar content
xerophillic organisms prefer low water availability
aldehydes found in oils of cinnamon natural antimicrobial agents
phenol found in oils of cloves - euglenol natural antimicrobial agents
benzoic acid cranberries natural antimicrobial agents
allicin antimicrobial sulfur compound found on garlic natural antimicrobial agents
lysozyme found in tears, egg yolk contains this; egg yolks are usually the ones to contaminate; spoilage of eggs by Proteus yields H2S natural antimicrobial agents
Polyphenols catechins found in unfermented green teas natural antimicrobial agents
Temperature lower temperature = reduce microbial growth and prolong storage
humidity reduce moisture = prolong storage
atmosphere shrink wrap allows for oxygen diffusion; increase oxygen leads to increased growth of microbes on surface
methods of prevention for food spoilage industrial canning, pasteurization, lyophilization, gamma irradiation
Industrial canning eliminates mesophilic bacteria and endospores hyperthermophllic microbes remainbut cannot grow at room temperatuer
pasteurization reduces number of microbes but not all die; used with delicate food and beverages
lyophilization Food is frozen then vacuumed out are its ice crystals
gamma irradiation penetrates food and damages DNA of microbes
sodium nitrate GOOD - inhibits growth of Clostirdium botulinum,nitric acid + meat keeps meat red BAD - reacts with amines leads to carcinogens
GRAS generally recognized as safe
nisin naturally producing bacteriocin made by Lactococcus lactis
hops plant added in production of beer for flavoring
wort fermented into beer; liquid remaining after barley solid is removed
ale dark, S. Cervisiae, top fermenting yeast
lager light, S. Carlsbergensis, bottom fermenting yeast
Swiss Cheese Propionibacterium shermanii
Roquefort Cheese Penicillium roqueforti
rennin an enzyme added to help with coagulation of casein
soy sauce Aspergillus oryzae (fungi)+ wheat + soybeans
Koji name of fungus covered mixture
Moromi Koji and salt solution that sits for a year to ferment
Pediococcus soyae and Lactobacillus produce acids in production of soy sauce
Saccharomyces rouxii produces small amount of alcohol in production of soy sauce
intrinsic factors inherent properties of the food itself pH, presence of natural microbiota, physical structures, food composition, water activity
Created by: acho4
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