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UOP Microbiology
Lecture 24: Food Microbiology
| Question | Answer | |
|---|---|---|
| extrinsic factors | factors involved with the processing/ handling of food; not the food itself | temperature, humidity, pressure |
| perishable foods | tend to be nutrient rich, moist, and unprotected by covering (rind) | need to be refrigerated; Ex) milk |
| semi-perishable foods | can store in sealed container for months without spoiling; many fermented foods | |
| non-perishable foods | usually dry or canned foods that can be stored almost indefinitely without spoiling | |
| high protein food spoilage | proteolysis/putrefacation, release smelly amines like cadaverines | |
| high fat food spoilage | lipid hydrolysis | rancid fatty acids |
| high carbohydrate spoilage | first signs indicated by fungi, not smelly | |
| Erwinia carotovora | cause fruits and vegetables to soft rot because it produces hydrolytic enzymes | |
| low pH | favors molds or yeasts | Ex) orange |
| high pH | favors bacteria | Ex) meat = alkaline |
| water activity | arbitrary number assigned on how moist something is | |
| osmophillic | organisms can tolerate high salt/ sugar content | |
| xerophillic | organisms prefer low water availability | |
| aldehydes | found in oils of cinnamon | natural antimicrobial agents |
| phenol | found in oils of cloves - euglenol | natural antimicrobial agents |
| benzoic acid | cranberries | natural antimicrobial agents |
| allicin | antimicrobial sulfur compound found on garlic | natural antimicrobial agents |
| lysozyme | found in tears, egg yolk contains this; egg yolks are usually the ones to contaminate; spoilage of eggs by Proteus yields H2S | natural antimicrobial agents |
| Polyphenols | catechins found in unfermented green teas | natural antimicrobial agents |
| Temperature | lower temperature = reduce microbial growth and prolong storage | |
| humidity | reduce moisture = prolong storage | |
| atmosphere | shrink wrap allows for oxygen diffusion; increase oxygen leads to increased growth of microbes on surface | |
| methods of prevention for food spoilage | industrial canning, pasteurization, lyophilization, gamma irradiation | |
| Industrial canning | eliminates mesophilic bacteria and endospores | hyperthermophllic microbes remainbut cannot grow at room temperatuer |
| pasteurization | reduces number of microbes but not all die; used with delicate food and beverages | |
| lyophilization | Food is frozen then vacuumed out are its ice crystals | |
| gamma irradiation | penetrates food and damages DNA of microbes | |
| sodium nitrate | GOOD - inhibits growth of Clostirdium botulinum,nitric acid + meat keeps meat red | BAD - reacts with amines leads to carcinogens |
| GRAS | generally recognized as safe | |
| nisin | naturally producing bacteriocin made by Lactococcus lactis | |
| hops | plant added in production of beer for flavoring | |
| wort | fermented into beer; liquid remaining after barley solid is removed | |
| ale | dark, S. Cervisiae, top fermenting yeast | |
| lager | light, S. Carlsbergensis, bottom fermenting yeast | |
| Swiss Cheese | Propionibacterium shermanii | |
| Roquefort Cheese | Penicillium roqueforti | |
| rennin | an enzyme added to help with coagulation of casein | |
| soy sauce | Aspergillus oryzae (fungi)+ wheat + soybeans | |
| Koji | name of fungus covered mixture | |
| Moromi | Koji and salt solution that sits for a year to ferment | |
| Pediococcus soyae and Lactobacillus | produce acids in production of soy sauce | |
| Saccharomyces rouxii | produces small amount of alcohol in production of soy sauce | |
| intrinsic factors | inherent properties of the food itself | pH, presence of natural microbiota, physical structures, food composition, water activity |