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Micro Ch 3 TEX
Microbiology Chapter 3 Dr Tex
| Question | Answer |
|---|---|
| What are the 5 I's? | Innoculation, Isolation, Incubation, Inspection, Identification |
| Innoculate means? | Put it on media |
| Incubate means? | Grow it |
| Isolate means? | Get it by itself |
| Inspect means? | Ensure a pure culture |
| Identify means? | Figure out what it is |
| Media is classified according to what three properties? | Physical state, chemical composition, and functional type |
| Physical states of media are broken down into what three categories? | Liquid, semi-solid, and solid |
| Liquid media are what? | Water based solutions known as broths, milks, and infusions |
| Semi-solid media usually has a low percentage of what? | agar (1%) |
| Semi-solid media is useful for testing what? | motility |
| Culturing is used to do what? | Rule out organisms and move forward to identify what organism is present |
| Solid media usually has a high percentage of what? | agar (1-5%) |
| Which kind of agar will grow almost all human pathogens? | Blood agar |
| Chemical content is broken down into what two categories? | Synthetic media versus nonsynthetic/complex media |
| Synthetic media contain what? | PURE, known organic or inorganic compounds |
| Complex/enriched media contain what? | UNKNOWN or impure ingredients (like animal extracts) |
| What are the three FUNCTIONAL types of growth media? | Selective, Enriched, Differential |
| What is selective media? | It selects for SPECIFIC SPECIES of organisms. EX: salt agar selects for staph |
| What is differential media? | allows multiple TYPES of organisms to grow but is designed to display VISIBLE DIFFERENCES (show different reactions) among them (ex MacConkey agar) |
| What is enriched media? | it is used to grow fastidious bacteria, ex. blood agar |
| What is a Durham Tube? | A tube inside a tube, shows if an organism can make gas and what it's pH is. |
| What are the 5 types of microscopes? | Magnification, Optical, Resolution, Electron, Stains |
| What is gram staining? | differential staining(pos or neg) penetrate the cell wall differently and thus ID orgs. |
| When using an oil immersion lens, the objective must be _______________? | IN THE OIL |
| Oil does not change the ________________ it changes the ________________. | Magnification, resolution. |
| Optical microscopes have a magnification of what? | 2000X |
| A live stain is also called what? | wet mount |
| Phase-contrast provides what? | a 3-D view |
| Flourescent stains provide what? | UV radiation and visible light from the dye |
| Confocal lenses provide what? | 3-D images of the insides of orgs |
| Electron microscopes provide what? | a view of the organelles |
| Positives stains do what? | bind to the specimen |
| Simple staining is what? | using one color stain to determine the shape of the org |
| Negative stains do what? | do NOT bind to the specimen, but AROUND the specimen |
| Positive stains are what kinds of dye? | BASIC (positive charge). They bind NEGATIVE charge cells. |
| Negative stains are what kinds of dye? | ACIDIC (negative charge). They bind POSITIVE charge cells. |
| What is acid-fast staining? | A type of staining for forcing dye through difficult cell walls, such as Tb cells, whose membrane is waxy |
| Medium | nutrient used to grow an organism |
| Agar | a polysaccaride found in seaweed, common media preparation |
| Colonies | macroscopic clusters of cells visible on media |
| Heat fixing | emulsifying an organism in a drop of water and then heating it on a slide |
| Flagella staining | uses basic stain and identifies proteus commonly found in UTIs |
Created by:
elizabethlaughlinlee
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