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Biology Lab 101
| Question | Answer |
|---|---|
| What is the chemical equation of photosynthesis? | 6CO2+6H2O+LIGHT → C6H12O6+6O2 |
| What is cellular respiration? | The break down of sugar and oxygen into carbon dioxide and water. |
| What are the aerobic steps of cellular respiration? | o Glycolosis o Krebs Cycle o Electron Transport Chain |
| What are the anaerobic steps of cellular respiration? | o Glycolisis o Alcohol or Lactic acid becomes final electron acceptor |
| What is the major carbohydrate that is broken down in glycolysis ? | glucose |
| Where does glycolysis occur in the cell? | cytoplasm |
| What are the end products of glycolysis? | pyruvic acid |
| Does this process require oxygen? | no oxygen required |
| How many ATP are produced? | 2 ATP produced |
| describe what happens in each stage of mitosis | • Mitosis o Prophase • Chromosomes condense and are well confined • Nucleolus begins to disassemble • In animals, paired centrosomes move to poles • Kinetochore fibers show up o Metaphase • Chromosomes become arranged so that they are all in middl |
| What type of cells undergo mitosis? | somatic cells |
| What are the type of cells undergo meiosis? | gametes, sex cells |
| What are the products of mitosis | 2 identical daughter nuclei cells |
| What are the products of meiosis? | 4 haploid cells |
| ploidy | number of set of chromosomes within a cell or organism |
| haploid cells | a single set of chromosomes (n) |
| diploid cells | a cell with two sets of chromosomes (2n) |
| gametogenesis | Production of gametes via meiosis in animals |
| What are some different types of gametogenesis? | Spermatogenesis and oogenesis |
| Spermatogenesis | produces gametes called sperm |
| oogenesis | produces gametes called ova |
| gamete | sex cell |
| How are traits passed from parent to offspring? | DNA |
| genotype | genetic makeup |
| phenotype | observable traits |
| What is random assortment? | Each parental allele has a 50% chance of being in a certain gamete i.e. coin toss |
| Codominance | when both alleles are expressed ie blood typing |
| Incomplete dominance | one allele for a specific trait is not completely dominant over the other allele ie half and half red+white=pink |
| Simple dominance | one allele is completely dominant to the other |
| Know how to construct a punnett square. Be able to determine the probability of specific genotypes and phenotypes using a punnett square. | *show genotype ie (Bb) phenotype: Brown |
| Homozygous recessive | a simple phenotype that can only be expressed when present as both alleles (aa) |
| Homozygous dominant | same alleles in phenotype expressed (AA) |
| Heterozygous dominant | phenotype expressed as only one allele (Aa) |
| structure of DNA | right-handed double helix, nucleotide composed of a sugar phosphate backbone and attached bases, is connected to a complementary strand by hydrogen bonding between paired bases, adenine (A) with thymine (T) and guanine (G) with cytosine (C). |
| structure of RNA | single strand right-handed helix. It's backbone structure is made from ribose sugars, phosphate, and the four bases adenine, guanine, cytosine and uracil |
| complementary base pairing | Cytosine is paired w/ thymine Adenine is paired with guanineAdenine and guanine are pyrimidines Cytosine and thymine are purines |
| What chemical can be used to detect DNA ? | Diphenylamine |
| Know and understand the steps in the procedure of isolating DNA from Onion Cells | 1. Dice onion into small tubes 2. Weight 25g of onion, transfer to 250 ml beaker 3. Add 50ml homogenizing solution, incubate in a 60C water bath or 15 min. (this step softens cell walls, helps dissolve cell membranes and denatures proteins that interfer |
| What reagents are used and how do they contribute to isolating DNA? | Sodium Chloride, Detergent ( SDS) and EDTA are used to lyse cell walls and membranes. Ethanol is added to precipitate DNA Diphenylamine used to test DNA Stored In Ethanol or dried and suspended in Tris-EDTA |
| onion cell | http://micro.magnet.fsu.edu/micro/gallery/mitosis/mitosis.html |
| White Fish- | http://www.instruction.greenriver.edu/kmarr/Biology%20211/Labs%20and%20ALEs/B211%20Labs/B211%20Labs/Onion%20and%20Whitefish%20Mitosis/Whitefish%20Mitosis/Whitefish%20Mitosis.htm |
| blood typing | http://learn.genetics.utah.edu/content/begin/traits/blood/ |