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DNA-Central Dogma

Test 3

QuestionAnswer
Holoenzyme core + sigma factor
Core Enzyme alpha, beta, beta prime, omega
What are the three steps in Replication, Transcription, Translation? Initiation, Elongation, Termination
Central Dogma Replication-Transcription-Elongation
How do the DNA strands separate at the replication fork? DnaA binds to FIVE 9bp segments at oriC
Who discovered DNA? Friedrich Miescher 1869
cAMP interacts with CRP; it regulates 100+ operons; also networks in phages such as lambda.
DNA gyrase enzyme that temporarily breaks the strands of DNA, relieving the tension cause by unwinding the two strands of the DNA helix.
DNA ligase Enzyme that joins two DNA fragments by forming covalent bond between the sugar-phosphate residues of adjacent nucleotides.
Helicases Enzymes that unwind the DNA helix ahead of the replication fork.
Okazaki fragments Nucleic acid fragment generated during discontinuous replication of the lagging strand of DNA.
DNA replication is generally bidirectional, semiconservative
DNA lagging strand Discontinuous, away from replication fork, 3-5
DNA leading strand Continuous, towards the replication fork, 5-3
Stop codons UAA, UAG, UGA
Anticodon complementary to an mRNA codon
Polyribosome/Polysome many ribosomes attached to a single mRNA molecule
Prokaryote Ribosome 30S + 50S = 70S
rRNA a type of RNA molecule found in ribosomes
Created by: 100000007429517
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