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Micro: Lab11; Ex.17

Antibiotic Resistance

QuestionAnswer
The most common chemically mediated controlling actions are... Biocidal action and biostatic action.
Biocidal action Killing activity
Biostatic action Growth arresting activity
An antimicrobial chemical agent with selective toxicity may be of therapeutic value in what? In treatment of infectious, or communicable diseases that are of a microbial orgin.
Biochemical agents; what does the prefix bio indicate? A chemical produced by a living organism.
Antibiotics are what? Biochemical agents.
What do antibiotics do? They exhibit selective toxicity to certain microbial life forms.
Difference between a narrow spectrum antibiotic and a broad spectrum antibiotic? A narrow spectrum antibiotic only exhibits toxicity to a few microbial organisms. A broad spectrum antibiotic exhibits toxicity to a relatively large, usually genetically diverse, group of microbes.
The first antibiotic agents were not identified and mass produced until when? Late 1930's early 1940's.
The mode of action refers to what? How the chemical agent actually inhibits the microbial growth and/or kills the organism.
Viable Alive
Metabolic processes that work as common targets which have been identified as sites for mode of action antibiotics? Cell wall synthesis, protein synthesis, RNA synthesis, DNA synthesis, and membrane transport.
Two techniques which evaluate the effectiveness of a particular antibiotic; Test tube dilution method and the agar diffusion method.
Agar diffusion method Kirby-Bauer test
What does the test tube dillution method do? It determines the lowest concentration of an antibiotic that will inhibit growth of a bacterium.
The lowest concentration on an antibiotic that will still inhibit growth of a bacterium is called... The minimal inhibitory concentration (MIC).
Kirby-Bauer test- A rapid procedure for determining the degree of sensitivity or resistance to a number of antibiotics.
The area where the antibiotic is at a high enough concentration to prevet growth- The zone of inhibition.
A zone of no growth- The Zone of inhibition.
What agar will be used? Mueller-Hinton agar.
What should you do at all times? (FOUR) Serilize the forceps between each application, space 4 discs evenly on a plate, and record the designation of each disc for referral.
Created by: kimblekn
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