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transport of membran

QuestionAnswer
what is the secretory pathway also referred to? exocytosis
What is a guaranteed pathway? ER -> budding vesicle -> cis-golgi network
What are the 3 types of protein coats? COPI, COPII, clathrin
Where is COPI localized to? cisternae of golgi
Where is COPII localized to? ER
Where is clathrin localized to? Golgi all the way to the plasma membrane
COPII mediated budding starts with what g protein? name some unique properties It is the inactive soluble SarI-GDP. It has a hidden amphipathic helix.
How does SarI get into the ER membrane? It's attached GDP turns into GTP by the sar1-GEF. It then exposes its amphipathic helix and imbeds itself into the membrane
What binds to the sar1-GTP? Sec24 and Sec23 and a cargo receptor
What lastly binds to the Secs and Sar1? Sec 13/31, forming a coat
What is a requirement for cargo proteins? They must be folded correctly to bind. Otherwise they go to chaperone proteins
When a vesicle is near its target, what happens first? Sar1-GTP hydrolyzes and loses its coat
A vesicle has what two factors attached to its outer membrane? v-SNARE and Rab-GTP
What is the significance of a rab-GTP on a vesicle? What does it interact with? What happens subsequently? Rab-GTP finds vesicles and binds to the rab effector. This binding allows for the vesicle to be localized to the cell surface
What is the significance of a v-SNARE on a vesicle? What does it interact with? What happens subsequently? v-SNARE fuses with t-SNAREs on the cell surface to form a 4-helix bundle at which fusion of the two membranes can begin
What are the three classifications of N-linked glycosylation? 1. complex oligosaccharide 2. high mannose 3. hybrid oligosaccharide
Where does O-linked glycosylation occur? In the golgi, and on a threonine or serine residue
What is the difference between vesicular transport and cisternal maturation models? vesicular transport - vesicles move, but nothing else cisternal - stacks shift placement and can become different regions of golgi
What are three different destinations for vesicles from the trans golgi network? 1. lysosomes 2. secretory vesicles 3. constitutive secretory pathway
What complex is associated with lysosome exiting? mannose-6
What is unique about vesicles going to the early endosome? clathrin coat
Created by: neeck
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