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Bio 111 Exam-3

all the vocab from the third exam

QuestionAnswer
Nucleotides Made up of 5 Carbon Sugar + Phosphate +Nitrogenous Base
Purines Bases with two rings (Adenine, Guanine)
Pyrimidines Bases with one ring (Cytosine, Uracil, Thymine)
Phosphodiester Bonds What connects nucleotides on the same strand
Hydrogen Bonds What connects nucleotides on opposite strands (easy to pull apart)
Semi-conservative Each old strand of DNA acts as a template for a new strand of DNA
Bidirectional Can go both ways
Origin of Replication A gap where DNA replication proceeds out from
Helicase Unwinds double stranded DNA
Topoisomerase Unwinds double stranded DNA and introduces temporary single-stranded nicks in DNA to unkink it. Also called “swivelase” makes a cut in a strand of DNA, releases DNA, DNA swivels, unwinds, removes kinks, seal is repaired
Single-stranded DNA Binding Protein Binds to unwound single stranded DNA and prevents it from reforming the double helix
Primer Short piece of DNA, act as starters for DNA Synthesis
Primase Makes primers
DNA Polymerase 3 Main enzyme involved in making DNA, makes DNA on the leading and lagging strands
Exonuclease Activity Used by DNA Polymerase 3 , breaks down nucleic acids, removes mismatched bases, proofreader
DNA Polymerase I Removes RNA Primer, fills in gap with DNA
Ligase Connects pieces of DNA together by making last phosphodiester bond
Chromatin Type of DNA, Complex of DNA and proteins, less folded, uncondensed, partially unwound
Chromosome Type of DNA, Complex of DNA and proteins, most toghtly packed, condensed in mitosis and meiosis
Nucleosome DNA wrapped around core of proteins
A is the promoter Region that controls when, where DNA is transcribed into RNA, in front of coding region
B-F Coding region part of gene that’s transcribed into RNA and then translated into protein
B, D, F Exons Regions that code for amino acids
G Termination Signal Sequence of DNA that tells RNA Polymerase to stop making RNA
Mrna (Messenger RNA) Codes for amino acids in proteins
Rrna Structural part of a ribosome
Ribosome Organelle, but it has a structural rrna as part of the ribosome
Trna(Transfer RNA) Brings amino acids to the ribosome during protein synthesis
Template Strand strand of DNA copied into RNA
Coding strand Strand of DNA that has same sequence as RNA
Transcription makes a pre-mRNA (exons + introns)
RNA Splicing Removes introns, makes mature mRNA
Cap 7 Methyl-G cap, added after transcription starts, before RNA synthesis is completed
Termination Signal Sequence in DNA, Makes RNA synthesis stop
SnRPS (RNA-protein particle) bind with other proteins to the intron and remove it
Ribosome Organelles where protein synthesis occurs, made of rrna and proteins
Codon Group of 3 nucleotides
Gene Segment of DNA that codes for protein, contains all information needed for a protein
Gene Regulation Process when a cell controls whether an active protein is made from a gene
Gene Expression Process when a gene is transcribed into RNA, then translated into protein, and the protein is converted to the active form of the protein
Constitutive Gene Expression The gene is expressed at the same level all of the time, in all cell types
Cell differentiation When a cell becomes a specialized cell type
Regulation of RNA Splicing In Eukaryotes, controls whether pre-mRNA is processed to a functional mRNA
Alternative splicing In Eukaryotes can get 2 different forms of protein from 1 pre-mRNA
Transcription Factor Protein that binds to DNA near the promoter of a gene and controls whether the gene is transcribed into RNA, alternate between active and inactive form
Repressors Type of Transcription factor, proteins that bind to the promoter of a gene and prevent RNA synthesis
Activators Proteins that bind near the promoter of a gene and increase or promote RNA synthesis
Effector molecule Small molecule that binds to the transcription factor and changes its shape (Allosteric regulation
Transcription Factor Protein that binds to specific DNA sequences, and thus controlling the transcription of DNA to RNA
Effector molecule Small molecule that binds to the transcription factor and changes its shape (Allosteric regulation, binds to allosteric site)
Operon Bacterial genes involved in the same process or pathway are sometimes clustered together into these. They are under the control of the same promoter.
Promoter Controls when genes in the operon are transcribed into RNA, includes the CAP site, the lac promoter and the operator
Structural Genes Many genes all controlled by the one promoter. Each gene codes for a different enzyme or protein involved in the process.
Polycistronic RNA This is one mRNA that contains the information to make a number of different proteins.
Inducible operons Work if a certain effector molecule is present; usually code for genes involved in breakdown pathways (catabolic pathways)
Repressible operons Repressed if a certain effector molecule is present, are usually involved in synthesis pathways (anabolic pathways)
lacZ codes for the enzyme b-galactosidase
lacY codes for lactose permease, transport protein
lacA codes for enzyme lactose acetylase
Catabolite Repression Allows bacteria to adapt quickly to a preferred carbon energy source first , shows one preferred energy source
Missense Changes one amino acid
Silent Mutation No Change
Nonsense changes regular codon to a stop codon
Frameshift Addition or deletion of one or more nucleotides in DNA, results in non-functional protein (Insertion or deletion)
Germ-like cells Pre-sex cells, makes gametes (sperm or egg), mutations in these cells are passed on
Somatic Cells Body cells (non-reproductive cells) , mutations in these cells are NOT passed on
Isomerization Changes in nucleotide structure
Transposons Source of mutation, a jumping gene, can have thousands of nucleotides, produces non-functional proteins
Induced Mutations Caused by environmental agents
Nucleotide Excision Repair Function is to remove abnormal bases from DNA (like thymine dimers, Uracil, other strange bases)
Xeroderma Pigmentosa When an individual is deficient in an enzyme needed for DNA repair
Sickle-Cell Anemia Caused by proteins clumping, making clumpy hemoglobin fibers, results from a missense mutation in the beta-globin gene
Created by: smkenned
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