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Medicine from Milk

Human Bio Exam 3

QuestionAnswer
What is a Protein? A macromolecule made up of repeating subunits known as amino acids, which determine the shape and function of a protein.
What are the functions of Proteins? 1. they allow our muscles to contract 2. give our hair and skin its texture 3. facilitate thousands of chemical reactions in our cells
How are genes expressed? When the cell uses the DNA instructions to make proteins.
What do chromosomes consist of? Many genes along their length.
Genotype An organism is the genetic make-up of that organism
Phenotype The physical attributes of the organism, including observable and non-observable traits.
What are antithrombins? Proteins that help prevent blood clots (thrombosis)
What does it mean if people inherit antithrombin deficiency? It means that both of their copies of the antithrombin gene are defective.
What are alleles? They are alternative versions of the same gene that have different nucleotide sequences.
What are the two parts that genes of how genes can be organized? 1. regulatory sequences determine when and how much protein a gene makes 2.Coding sequences determine the amino acid sequence of the encoded protein
What is gene therapy? A type of treatment that aims to cure disease by replacing defective genes with functional ones.
What is the first step of making a transgenic goat? Create a hybrid gene; goat regulatory sequence and human antithrombin coding sequences are cut out of donor cell chromosomes and joined together using special enzymes.
What is the second step of making a transgenic goat? Microinjection and embryo transfer
What is the third step of making a transgenic goat? purify antithrombin from transgenic milk.
Two main steps of gene expression Transcription and Translation
Transcription occurs where in eukaryotic cells? prokaryotic cells? E= nucleus P= cytoplasm
Translation occurs where? Ribosomes: are the cellular machinery used for protein synthesis
Transcription The first stage of gene expression Copies the coding sequence of DNA into the complementary messenger RNA (mRNA) sequence.
Translation the second stage of gene expression Uses the mRNA sequence to assemble the appropriate amino acid sequence of the protein
Transgenic Refers to an organism that carries one or more genes from a different species
Gene A sequence of DNA that contains the information to make at least one protein
Coding Sequence The part of a gene that specifies the amino acid sequence of a protein. Coding sequences determine the identity, shape, and function of protein.
mRNA The RNA copy of an original DNA sequence made during transcription
RNA Polymerase The enzyme that accomplishes transcription. RNA polymerase copies a strand of DNA into a complementary strand of mRNA
Codon A sequence of three mRNA nucleotides that specifies a particular amino acid.
Transfer RNA A type of RNA that helps ribosomes assemble chains of amino acids during translation
Anticodon the part of a tRNA molecule that binds to a complementary mRNA codon.
Genetic Code The particular amino acids specified by particular mRNA codons.
Created by: sbarne12
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