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CT
CT exam 2
| Question | Answer |
|---|---|
| Masson Trichrome stain differentiates between what? (2) | Smooth Muscle and Collagen in tumors |
| Masson Trichrome is also used to identify what? | Increases in collagenous tissue (disease) |
| What is the preferred fixative in Masson Trichrome? | Bouin |
| What are good controls for Masson Trichrome Stain? | Uterus, Fallopian tubes, Small intestine, Appendix |
| What color will the nuclei stain? | Black due to the Weigerts Hematoxylin |
| What color will the cytoplasm, keratin, muscle fibers stain? | Red due to the Beibrich Scarlet-Acid Fushin |
| What color will the collagen and mucus stain? | Blue due to the aniline blue |
| In Masson Trichrome, Decreased red staining is indicative of what? | Depleted stain |
| In Massin Trichrome. Decreased blue could mean? | over-differentiated with Aceted Acid |
| What does Gomori One Step trichrome differentiate? | Smooth muscle and collagen or an increase of collagen |
| What is the mordant of the Gomori one step? and when is it supposed to be fixed? | Bouin and it is a mordant prior to staining |
| What are controls for Gomori? | Appendix, Uterus, Small Intestine |
| What are the colors for nuclei/cytoplasm , keratin, muscle fibers-/Collagen and mucus | black , red , blue |
| In Van Geison, collagen is stained by? | Acid Fuchsin |
| Van Geison, What does Picric acid provide and stain? | provides acid PH and stains muscle and cytoplasm |
| What will the nuclei, collagen, muscle and cytoplasm stain? | black, brilliant red, yellow |
| Elastic fibers are selective for what kind of dye compounds? | Basic |
| True or false,in elastic stains, all fixatives are compatible | False, most fixatives are |
| In Verhoeff Elastic Stain, what does this demonstrate? | pathological changes in elastic fibers or for identification of elastic |
| What is a good control for Verhoeff? | Artery |
| Verhoeff uses what type of stain? regressive or progressive | Regressive |
| What is the function of ferric chloride in Verhoeff? | Mordant and oxidizer |
| What is the counterstain for Verhoeff? | Van Gieson |
| Elastic has the strongest affinity for what type of hematoxylin? | Iron and will retain dye longer |
| What does Sodium thiosulfate remove? | excess iodine |
| In elastic staining solutions, when must the staining solutions be prepared? | in the order given |
| What is the counterstain for aldehyde fuchsin? | Light green |
| What fixatives do you avoid in Aldehyde fuschin | Chromate |
| In Orecin, What will the elastic fibers stain? | brown |
| What does Movat Pentachrome demonstrates? | mucin, fibrin, elastic fibers, muscle and collagen |
| Silver Stains demonstrates what? | Reticular fibers patterns |
| Gomori for reticular fibers, What is the oxidizer? Sensitizer?Reducer? Toner? | oxidizer-potassium permanganate, sensitizer-ferric ammomium sulfate, reducer-formalin, toner-gold chloride |
| Gordon and Sweets, What is the oxidizer and sensitizer? | oxidizer-potassium permanganate/sensitizer- ferric ammonium sulfate |
| In Snooks, what would cause granular staining? | old reagents |
| What does PTAH demonstrate? | Cross striations and fibrin |
| What does PTA bind to and what does it give? | PTA binds to hematein and gives a blue color lake. |
| What does color does PTA stain? | Red brown |
| What is the preferred fixative for PTAH? | Zenker |
| PTAH solution must be naturally..? What happens if you use potassium permangante? | Ripen, adding potassium permangante will give the solution short shelf life |
| In PTAH, what happens if tissues are not fixed in Zenkers? | uneven staining |
| What is the purpose of sodium thiosulfate? | removes mercury and excess iodine |
| What bleaches potassium permanganate? | oxalic acid |
| What are the results for PTAH?Cross striations?Nuclei, Collagen? | Cross Striations-Blue, Nuclei-blue, Collen red brown |
| What type of stain stains basement membrane? | Carbohydrate stains |
| What are the functions of Basement Membrane? | Ultrafilration, Cell attachment, supports epithelium |
| In PMS, Carbs are oxidized to..? | Aldehydes |
| Silver ions are bound to ..... and then reduced ti visible metallic silver by the.... | carbohydrates, aldehydes |
| What is the oxidizer in PMS? | Periodic acid |
| What stain is used for Mast Cells? | Toluidine Blue |
| How does Mast cells stain with Toludine Blue? | Metachromatically, they stain a different color from the dye and the rest of the tissue |
| What stain is used to stain Plasma Cells? | Methyl Green Pyronin Y |
| In Methyl Green-Pyronin Y , What part stains DNA?RNA? | Methyl Green stain DNA, Pyronin stain RNA |
| What is fibrin? | Polymerization of fibrogen, a plasma protein |
| Where is fibrin commonly seen? | inflammatory tissue damage |
| What stain stains fibrin? | Lendrum MSB |
Created by:
alexmcarpio
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