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Bio251 Lab Practical

Microbiology Biol 251 Lab Practical #1

QuestionAnswer
Ocular lenses magnify 10 or 15x
Objectives Grouped in revolving nosepiece. Scanner X4 Low power X10 High power x40 Oil immersion X100
Condenser lens Located under the stage. Focuses and concentrates the light reaching the specimen.
Iris diaphragm within the condenser can be opened/closed with a lever to regulate the amount of light reaching the slide.
Stage Flat surface for placing slides
Stage clips Clips the hold slide in place.
Mechanical Stage Knobs that move the platform stage. slides side to side, front and back
Lamp switch On/off switch for light source
Rheostat Knob that regulates amount of light leaving the lamp
Coarse adjustment knob Large knob to use with 4x and 10x
Fine adjustment knob Small knob used for fine focus. 40x and 100x
Arm Metal area to hold microscope
Base Flat metal bottom for holding microscope for carrying.
Working Distance Distance between the objective lens and the slide when in focus
Resolution Ability to distinguish objects
Parfocal An image in focus will remain in focus after changing from low power to high power.
Name the 3 domains of microorganisms Archae, Bacteria, and Eukarya
Kingdoms in Eukarya 1. Protists 2. Fungi 3. Plantae 4. Animalia
Nucleus Prokaryotes NO Eukaryotes YES
DNA Prokaryotes single circular Eukaryotes multiple Linear
Membrane Prokaryotes Cell membrane Eukaryotes Cell and orgamelle membrane
Organelle Prokaryotes NO Eukaryotes yes
Ribosomes Prokaryotes Yes free ribosomes cytoplasm Eukaryotes Yes free and bound ribosome in ER and cytoplasm
Cytoskeleton Prokaryotes No Eukaryotes Yes
Cell wall Prokaryotes Yes Eukaryotes in fungi algae plants
Flagella Prokaryotes Yes Rotating Eukaryotes Yes whip like
Cilia Prokaryotes No Eukaryotes Yes
what is required for mold to grow 1. Mold spores 2. Mold food 3. Temperature 4. Moisture
How does yeast grow Fermentation, budding
Define Sterile Free from all living organisms
Define aseptic technique A way of working with microbial cultures that insures the environment personal and the microbial culture are not contaminated. Original culture remains pure.
Created by: kmmiller07
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