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MICB 3301 Lab Quiz 2

Staining and Fungi

QuestionAnswer
What is a general name for a benzene substituent that is responsible for creating color? Chromophore
What is a general name for a benzene substituent responsible for increasing solubility of the compound? Auxochrome
2 Important Basic Dyes Methylene Blue (Mainly stains Metachromatic granules or volutin granules (phosphate storage)) Crystal Violet
Purpose of Iodine in Gram Staining Mordant (Sets stain)
Bright red counterstain Safranin
Gram Positive Characteristics (Color stain, and composition) Stains Purple, thick peptidoglycan layer, techoic acids present, no LPS, no outer membrane
Gram Negative Characteristics Stains Pink/Red, thin peptidoglycan layer, no techoic acids, LPS and outer membrane present.
Average size of rod shaped bacterium 1 x 3 microns
Average size of a coccus shaped bacterium. 1 micron in diameter
Average size of a spiral shaped bacterium 0.5 x 15 microns
Capsule Composition polysaccharides or polypeptides
A capsule is usually indicative of what kind of bacteria? Pathogenic bacteria
Visualization method used to view capsules Negative Staining
Specimen used for capsule stain demonstration Klebsiella
Average size of a Flagella 20 nm
Mechanism of flagella staining Mordant precipitates onto flagella to increase its size.
Acid-Bacteria (Main Characteristic) Resistance of decolorization with acidified alcohol.
Protoplast Cell devoid of cell wall.
Reagent used to remove cell wall of certain bacteria. Lysozyme
Mechanism of Lysozyme Hydrolysis of the glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine
Two categories of fungi Yeasts and molds
Characteristic of Molds Multicellular, filamentous, branched networks
Characteristics of Yeast Unicellular, sexual reproduction through spores OR asexual reproduction via budding
Filament grown by fungi Hyphae
Network of hyphae mycelium
Hyphal cell wall is usually comprised of... chitin
Individual hyphae with regular cross-walls septate
Hyphae not interrupted by cross walls Aseptate (or nonseptate)
Spores that develop in sac-like structures at the end of their hyphae Sporangiospore
Spores that do not develop in a sac and are generally located at the tips/sides of hyphae Conidiospores (Conidia)
Fungi Subdivision: Simplest of fungi, forms motile zoospores with flagella, sexual and asexual reproduction. Implicated in massive killing of frog populations. Chytridiomycota
Fungi Subdivision: Common in soil and decaying plant material, aseptate hyphae, sexual and asexual reproduction, Rhizopus is a representative member. Zygomycota
Fungi Subdivision: Sac-like reproductive structures called ascus, major decomposers in many terrestrial environments, sexual and asexual reproduction. Includes Aspergillus and Saccharomyces cerevisiae Ascomycota
Fungi Subdivision: Club fungi, basidium, sexual and asexual reproduction. Includes Cryptococcus neoformans and mushrooms/toadstools. Basidiomycota
Created by: 1278840111
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