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lab quiz microbio 2
CU microbiology Lab Quiz 2
| Question | Answer |
|---|---|
| Experiment #9: Know which organism is positive for each test. "what are the tests" | startch- Bracillus cereus (w/ grams Iodin will be colorless not black) (detect proteolysis) gelatin- Pseudomonas aeruginosa (will be a liquid) (detects proteolysis)Milk- Bracillus cereus (clear in agar) (Fat usage)tributyrin agar- Pseudomonas aeruginos |
| To test for a culture to utilize starch is detected by...... | the addition of grams iodine in a culture grown in the presence of starch. Bracillus cereus is positieve |
| proteolysis- two methods to detect it.... | gelatin agar- culture digests gelatin to a liquid - Pseudomonas aeruginosa milk agar- digests casein and goes from milky white agar to clear: Bracillus cereus |
| Fat usage or lipolysis is dectected by | using a tributyrin agar will be cloudy but when cuture uses fat it is clear: Pseudomonas aeruginosa |
| Know results for Escherichia coli in experiment 10. Fermentation of Carbohydrates | *Lactose broth and Glucose broth had acid and gas reactions: The E. coli in the lactos broth had half purple and half yellow.... |
| Ex. 11 what are the cultural characteristics which can be determined. 1. lactose 2. proteolysis 3. Rennent coagulation 4. Reduction 5. Ropiness | 1.1 Acid -- Pink, milk - liquid 1.2 acid curd-- pink, acid coagulates milk protein 1.3acid and gas -- bubbles in the curd 2. blue, milk watery 3.blue, poteins are coagulatedby enzymes not acid 4. white usually at bottom. 5. excessibe capsule produc |
| Ex. 12 Know which organism produced hydrogen sulfide | Kliglar iron agar- proteus vulgarus or serratia marcescenes |
| Ex. 13 Know which organism produced indole know procedure step 3 | Tryptone broth turns a red color at the top of the broth means a positive test for the presence of indole. Proteus vulgaris. |
| Ex. 14 Know which organism reduced resazurin | Streptococcus lactis turns white/pink though pseudomonas aeruginosa has bad quality as well and is not successfuly tested for. |
| ex. 15 Know proceure steps 3-5 | Nitrate broth: Sulfanic Acid and a-Saphthylamine are added. This causes red, but if there is no reaction then we put Zinc in to prove that NO3 has not reduced |
| Ex. 16 know which organism produced urease | Urease broth: Proteus vulgaris |
| Ex. 17 Know which procedure steps 3, 4 | Select one tube of each organism. Test for Acid production - add methyl red. red color indicates a low pH and a positive methyl red test. 4. Add a dropper fulll of a-naphthol to other tubs. Ad dropper full of KOH to tubes. pink/red + acetylmethylcarbinol |
| Ex. 17 Know results for each organism | Escherichia coli and Enterobacter aerogenes E. coli is positive for Methyl red and negative for VP Enterobacter aerogenes is positive for VP and negative is Methyl Red. |
Created by:
jseekins
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