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Biology PP22
Transcription and Translation
| Question | Answer |
|---|---|
| RNA polymerase | enzyme that catalyzes the transcription reaction |
| promoter sequences determine what? | What, Where, and How Much the gene is transcribed |
| Where do RNA polymerase and transcription factors bind to? | promoter region |
| What bind a promoter? | RNA polymerase and transcription factors |
| activators | increase transcription |
| repressors | block transcription |
| Describe Transcription | (blank) |
| What does translation require? | tRNA, rRNA, mRNA, plus amnio acids and energy |
| what is ribosome made of? | mostly of rRNA + small proteins |
| are most enzyme proteins? | yes |
| what is the catalyst for translation? | ribosome |
| what does tRNA do? | bring amino acids to the ribosome |
| anticodon | complementary to the codon on mRNA |
| AUG | (initiation codon) marks the beginning of translation |
| promoter | region of DNA where transcription is initiated |
| explain elongation | tRNA goes to A site and its anticodon hooks to mRNA codon. Amino acid of tRNA in P site hooks on to 2nd tRNA; 1st tRNA unhooks and 2nd tRNA moves to P site |
| dehydration | take monomer and make polymer |
| explain termination | (blank) |
| What are the stop codons? | UAA, UAG, UGA |
| what are genes compose of? | exons and introns |
| exons | nucleotides sequence that are transcribed and translated |
| Introns | transcribed not translated |
| What's the percent of gene that is not translated? | 90% |
| Does bacteria and mitochondria have introns? | no |
| why do we need introns? | allow exons to mix and match for new functions. |
| what are pre-mRNA composed of? | exons and introns |
| Is 90% of a gene introns or extrons? | introns |
| how can different combinations of exons generate different protein? | through alternative splicing |
| 3 things that are done to mRNA before it leaving the nucleus | 1) splice introns (cut out) 2) put on 5' cap 3) 3' tail |
| 5'cap | help ribosomal subunit attach |
| tail | protect RNA |